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Marker-free, tissue-specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants

文献类型： 外文期刊

作者： Qi, Yongbin 1 ; Chen, Lei 1 ; He, Xiuling 3 ; Jin, Qingsheng 1 ; Zhang, Xiaoming 1 ; He, Zuhua 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Crops & Nucl Technol Utilisat, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Univ, Inst Biotechnol, Coll Agr & Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Serocultural Res Inst, Hangzhou, Zhejiang, Peoples R China

4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai, Peoples R China

关键词： Cry1Ab gene;rbcS promoter;Bt toxin;co-transformation;marker-free;tissue-specific expression

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2013 年 69 卷 1 期

页码：

收录情况： SCI

摘要： BACKGROUND: Rice is the major food resource for nearly half of the global population; however, insect infestation could severely affect the production of this staple food. To improve rice insect resistance and reduce the levels of Bt toxin released into the environment, the Cry1Ab gene was conjugated to the rice rbcS promoter to express Bt toxin in specific tissues of transgenic plants. RESULTS: Eight marker-free, T2 lines were separated from the T0 cotransformants. Using RT-PCR, high levels of Cry1Ab expression were detected in the leaf but not in the seed. The Cry1Ab protein level ranged from 1.66 to 3.31 mu g g-1 in the leaves of four transgenic lines, but was barely detectable in their seeds by ELISA. Bioassays showed that the mortality rate of silkworm larvae feeding on mulberry leaves dipped in transgenic rice flour and pollen was less than that of the positive control (KMD), and that their average weight was higher than that of KMD, suggesting that the Cry1Ab protein was not expressed in the seed and pollen. CONCLUSION: The transgene conferred a high level of resistance to insects and biosafety to the rice plants, which could be directly used in rice breeding. Copyright (c) 2012 Society of Chemical Industry

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