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Box-Behnken Design for Optimizing Ultrasonic-Assisted Enzymatic Extraction of Soluble Dietary Fiber from Pleurotus citrinopilestus

文献类型： 外文期刊

作者： Yu, Panling 1 ; Yu, Changxia 1 ; Chen, Mingjie 1 ; Dong, Qin 1 ; Hu, Die 1 ; Zhang, Baosheng 1 ; Zhang, Mengke 1 ; Ma, Jianshuai 1 ; Xu, Baoting 1 ; Zhao, Yan 1 ; Proestos, Charalampos 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

关键词： soluble dietary fiber; Pleurotus citrinopilestus; ultrasonic-assisted enzymatic extraction; response surface methodology; optimization

期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）

ISSN：

年卷期： 2023 年 9 卷 12 期

页码：

收录情况： SCI

摘要： Pleurotus citrinopilestus contains a variety of physiologically and pharmacologically active compounds. A key active component among these compounds is dietary fiber, a polysaccharide that exhibits several biological properties. The objective of this study was to assess how soluble dietary fiber (SDF) from Pleurotus citrinopilestus responded to ultrasonic-assisted enzymatic (UAE) extraction. The response surface method (RSM) combined with the Box-Behnken design method (BBD) was used to optimize the yield of SDF. The effects of the liquid-solid ratio (35-55 mL/g), alpha-amylase concentration (0.5-2.5%), complex protease concentration (0.4-2.0%), and ultrasonication time (15-55 min) on the yield of SDF were examined. The RSM results revealed the optimal liquid-solid ratio (45 mL/g), alpha-amylase concentration (1.5%), complex protease concentration (1.2%), and ultrasonic time (35 min). The SDF yield was 10.25%, which is close to the predicted value (10.08%).

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