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Homologous Delta-12 Fatty Acid Desaturase (FAD2) Genes Affect Gene Expression and Linoleic Acid Levels in Lentinula edodes under Heat Stress

文献类型： 外文期刊

作者： Yang, Huanling 1 ; Jiang, Jun 2 ; Chen, Mingjie 1 ; Song, Xiaoxia 1 ; Yu, Changxia 1 ; Chen, Hongyu 1 ; Zhao, Yan 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China

2.Lishui Inst Agr & Forestry Sci, Lishui 323000, Peoples R China

关键词： Lentinula edodes; delta-12 fatty acid desaturase; differential gene expression; heat stress; bioinformatic analysis

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 7 期

页码：

收录情况： SCI

摘要： Delta-12 fatty acid desaturases (FAD2s) actively regulate stress responses and cell differentiation in living organisms. In this study, six homologous FAD2 genes were identified based on the genome sequence of Lentinula edodes. Then, the six FAD2 protein sequences were analyzed using bioinformatics tools, including ExPASy ProtParam, SignalP, TMHMM, and TargetP. These analyses were performed to predict the physical and chemical properties, signal peptides, and transmembrane and conserved domains of these proteins. The polypeptide sequences were aligned, and a maximum likelihood phylogenetic tree was constructed using MEGA 7.0 software to elucidate the phylogenetic relationships between homologous FAD2 sequences. The results demonstrated that the FAD2 proteins contained three conserved histidine-rich regions (HXXXH, HXXHH, and HXXHH), which included eight histidine residues. The linoleic acid content and FAD2 enzyme activity were further analyzed, and the levels in the mutagenic heat-tolerant strain 18N44 were lower than those in the wild-type strain 18. Interestingly, the expression levels of the FAD2-2 and FAD2-3 genes under heat stress in strain 18N44 were lower than those in strain 18. These findings indicated that FAD2-2 and FAD2-3 may play major roles in the synthesis of linoleic acid during heat stress.

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