您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Mycoreovirus genome alterations: similarities to and differences from rearrangements reported for other reoviruses

文献类型： 外文期刊

作者： Tanaka, Toru 1 ; Eusebio-Cope, Ana 1 ; Sun, Liying 2 ; Suzuki, Nobuhiro 1 ;

作者机构： 1.Okayama Univ, Inst Plant Sci & Bioresources, Agrivirol Lab, Kurashiki, Okayama 7100046, Japan

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Beeding Base Zhejiang Sustainble Pe, Key Lab Biotechnol Plant Protect,Minist Agr, Hangzhou 310021, Zhejiang, Peoples R China

关键词： reovirus;rearrangement;mycoreovirus;Cryphonectria parasitica;chestnut blight;hypovirus;papain-like protease p29;dsRNA

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2012 年 3 卷

页码：

收录情况： SCI

摘要： The family Reoviridae is one of the largest virus families with genomes composed of 9-12 double-stranded RNA segments. It includes members infecting organisms from protists to humans. It is well known that reovirus genomes are prone to various types of genome alterations including intragenic rearrangement and reassortment under laboratory and natural conditions. Recently distinct genetic alterations were reported for members of the genus Mycoreovirus, Mycoreovirus 1 (MyRV1), and MyRV3 with 11 (S1-S11) and 12 genome segments (S1-S12), respectively. While MyRV3 S8 is lost during subculturing of infected host fungal strains, MyRV1 rearrangements undergo alterations spontaneously and inducibly. The inducible MyRV1 rearrangements are different from any other previous examples of reovirus rearrangements in their dependence on an unrelated virus factor, a multifunctional protein, p29, encoded by a distinct virus Cryphonectria parasitica hypovirus 1 (CHV1). A total of 5 MyRV1 variants with genome rearranged segments (S1-S3, S6 and S10) are generated in the background of a single viral strain in the presence of CHV1 p29 supplied either transgenically or by coinfection. MyRV1 S4 and S10 are rearranged, albeit very infrequently, in a CHV1 p29 independent fashion. A variant of MyRV1 with substantial deletions in both S4 and S10, generated through a combined reassortment and rearrangement approach, shows comparable replication levels to the wild-type MyRV1. In vivo and in vitro interactions of CHV1 p29 and MyRV1VP9 are implicated in the induction of MyRV1 rearrangements. However, the mechanism underlying p29-mediated rearrangements remains largely unknown. MyRV1 S4 rearrangements spontaneously occurred independently of CHV1 p29. In the absence of reverse genetics systems for mycoreoviruses, molecular and biological characterization of these MyRV1 and MyRV3 variants contribute to functional analyses of the protein products encoded by those rearranged segments.

  • 相关文献

[1]Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction. Eusebio-Cope, Ana,Suzuki, Nobuhiro,Sun, Liying,Hillman, Bradley I.. 2010

[2]The Complete Genome Sequence of Two Isolates of Southern rice black-streaked dwarf virus, a New Member of the Genus Fijivirus. Wang, Qiang,Zhou, Guo-Hui,Yang, Jian,Zhang, Heng-Mu,Chen, Jian-Ping,Adams, Michael J..

[3]Whole blood transcriptome comparison of pigs with extreme production of in vivo dsRNA-induced serum IFN-a. Liu, Xiangdong,Huang, Jing,Yang, Songbai,Zhao, Yunxia,Xiang, Anjing,Cao, Jianhua,Fan, Bin,Zhao, Shuhong,Zhu, Mengjin,Liu, Xiangdong,Huang, Jing,Yang, Songbai,Zhao, Yunxia,Xiang, Anjing,Cao, Jianhua,Fan, Bin,Zhao, Shuhong,Zhu, Mengjin,Liu, Xiangdong,Zhao, Junlong,Wu, Zhenfang,Huang, Jing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台