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Modulation of Exogenous Glutathione in Ultrastructure and Photosynthetic Performance Against Cd Stress in the Two Barley Genotypes Differing in Cd Tolerance

文献类型: 外文期刊

作者: Wang, Fang 1 ; Chen, Fei 1 ; Cai, Yue 1 ; Zhang, Guoping 1 ; Wu, Feibo 1 ;

作者机构: 1.Zhejiang Univ, Dept Agron, Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Crop Sci & Nucl Tech Applicat, Hangzhou 310021, Zhejiang, Peoples R China

关键词: Cadmium (Cd);GSH;Barley (Hordeum vulgare L.);Photosynthesis;Ultrastructure;Genotypic difference

期刊名称:BIOLOGICAL TRACE ELEMENT RESEARCH ( 影响因子:3.738; 五年影响因子:3.44 )

ISSN: 0163-4984

年卷期: 2011 年 144 卷 1-3 期

页码:

收录情况: SCI

摘要: Greenhouse hydroponic experiments were conducted using Cd-sensitive (Dong 17) and tolerant (Weisuobuzhi) barley genotypes to evaluate genotypic differences in response of photosynthesis and ultrastructure to Cd toxicity in the presence of exogenous glutathione (GSH). Addition of 20 mg L(-1) GSH in 5 mu M Cd culture medium (Cd + GSH) significantly alleviated Cd-induced growth inhibition and reduced Cd concentration in leaves and roots especially in the sensitive genotype Dong 17. Exogenous GSH greatly ameliorated Cd-induced damages on leaf/root ultrastructure, e.g., compared with Cd alone treatment, chloroplasts in plants treated with Cd + GSH become better or in relatively normal shape with well-structured thylakoid membranes and parallel pattern of lamellae and unfolded more starch grains but less osmiophilic plastoglobuli; nuclei of root cells were better formed and chromatin distributed more uniformly in both genotypes, and number of plastids and mitochondria cristae in Dong 17 resumed to control level. The examination of photosynthetic performance revealed GSH dramatically increased net photosynthetic rate (P (n)), stomatal conductance (G (s)), and transpiration rate (T (r)) in the both genotypes and strongly stimulated Cd-induced decrease in the maximal photochemical efficiency (F (v)/F (m)) especially in the sensitive genotype.

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