文献类型： 外文期刊
作者： Xie, Xiaobo 1 ; Qiu, Yingying 1 ; Ke, Liping 2 ; Zheng, Xiliang 1 ; Wu, Guanting 2 ; Chen, Jinqing 2 ; Qi, Xingjiang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
3.Chungnam Natl Univ, Dept Agron, Taejon 305764, South Korea
关键词： ISSR-suppression PCR method;microsatellites;Myrica rubra;red bayberry
期刊名称：AMERICAN JOURNAL OF BOTANY （ 影响因子：3.844； 五年影响因子：3.657 ）
ISSN： 0002-9122
年卷期： 2011 年 98 卷 4 期
页码：
收录情况： SCI
摘要： Premise of the study : Microsatellite markers were developed in Myrica rubra to investigate potential hybridization events within or between M. rubra and its closely related species. Methods and Results : Using an ISSR-suppression PCR method, 12 primer pairs were temporarily developed with GSG(GT)(6) as the primer for enriching microsatellite sequences and the genomic DNA of M. rubra cv. 'Heijing' as template. The average allele number per locus was 4.9 within 26 individuals including two species, M. rubra and M. nana. Three pairs of primers produced species-specific alleles, and the other nine showed polymorphisms among 26 accessions. Conclusions : Results indicated that the ISSR-suppression PCR method is suitable for developing microsatellite markers, especially for this species with little understanding of genomic information. The developed microsatellite markers provide a useful tool for further studies of population structure within or between M. rubra and M. nana or other closely related species.
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