文献类型： 外文期刊

作者： Xiao, C. ¹ ; Bao, G. ² ; Hu, S. ¹ ;

作者机构： 1.Zhejiang Univ, Coll Anim Sci, Dept Vet Med, Hangzhou 310029, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： extract of Momordica cochinchinensis (Lour.) Spreng. seeds;adjuvant;saponin;vaccine;Newcastle disease

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

ISSN： 0032-5791

年卷期： 2009 年 88 卷 11 期

页码：

收录情况： SCI

摘要： The study evaluated the immunological effect of extract of Momordica cochinchinensis (Lour.) Spreng. seeds (ECMS) on the immune response against Newcastle disease (ND) in chickens. Forty-eight chickens were divided into 4 groups (n = 12). Each chicken was immunized with ND vaccine mixed with 0, 20, 40, or 80 mu g of ECMS on d 35. Blood samples were collected on d 0, 7, 14, 21, 28, and 35 postimmunization. Humoral and cellular immune responses were evaluated by indirect ELISA assay and the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Results indicated that humoral immune response was enhanced by ECMS 14 d postimmunization. Eighty micrograms of ECMS was the best dose with the ND vaccine and was significantly different from the other groups 21 d after immunization. No significant differences were found in the cellular immune response, whereas the 80 mu g of ECMS group had higher values than the other groups 35 d after immunization. No side effect was found on the growth performance during the experiment.