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bra-miR167a Targets ARF8 and Negatively Regulates Arabidopsis thaliana Immunity against Plasmodiophora brassicae

文献类型： 外文期刊

作者： Liao, Rujiao 1 ; Wei, Xiaochun 1 ; Zhao, Yanyan 1 ; Xie, Zhengqing 2 ; Nath, Ujjal Kumar 3 ; Yang, Shuangjuan 1 ; Su, Henan 1 ; Wang, Zhiyong 1 ; Li, Lin 1 ; Tian, Baoming 2 ; Wei, Fang 1 ; Yuan, Yuxiang 1 ; Zhang, Xiaowei 1 ;

作者机构： 1.Zhengzhou Univ, Inst Hort, Henan Acad Agr Sci, Grad T&R Base, Zhengzhou 450002, Peoples R China

2.Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improvem, Zhengzhou 450001, Peoples R China

3.Bangladesh Agr Univ, Dept Genet & Plant Breeding, Mymensingh 2202, Bangladesh

关键词： Chinese cabbage; clubroot; phytohormone; miR167a; auxin

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 14 期

页码：

收录情况： SCI

摘要： Clubroot is a soil-borne disease caused by Plasmodiophora brassicae, which can seriously affect the growth and production of cruciferous crops, especially Chinese cabbage crops, worldwide. At present, few studies have been conducted on the molecular mechanism of this disease's resistance response. In this experiment, we analyzed the bioinformation of bra-miR167a, constructed a silencing vector (STTM167a) and an overexpression vector (OE-miR167a), and transformed them to Arabidopsis to confirm the role of miR167a in the clubroot resistance mechanism of Arabidopsis. Afterwards, phenotype analysis and expression level analysis of key genes were conducted on transgenic plants. From the result, we found that the length and number of lateral roots of silence transgenic Arabidopsis STTM167a was higher than that of WT and OE-miR167a. In addition, the STTM167a transgenic Arabidopsis induced up-regulation of disease resistance-related genes (PR1, PR5, MPK3, and MPK6) at 3 days after inoculation. On the other hand, the auxin pathway genes (TIR1, AFB2, and AFB3), which are involved in maintaining the balance of auxin/IAA and auxin response factor (ARF), were down-regulated. These results indicate that bra-miR167a is negative to the development of lateral roots and auxins, but positive to the expression of resistance-related genes. This also means that the STTM167a can improve the resistance of clubroot by promoting lateral root development and the level of auxin, and can induce resistance-related genes by regulating its target genes. We found a positive correlation between miR167a and clubroot disease, which is a new clue for the prevention and treatment of clubroot disease.

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