Establishment of an efficient root mediated genetic transformation method for gene function verification in citrus
文献类型： 外文期刊
作者： Xiao, Yu-Xiong 1 ; Dutt, Manjul 2 ; Ma, Hai-jie 3 ; Xiao, Cui 1 ; Tong, Zhu 1 ; Wang, Ze-Qiong 1 ; He, Xiu-Juan 1 ; Sun, Zhong-Hai 1 ; Qiu, Wen-Ming 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Fruit & Tea, Hubei Key Lab Germplasm Innovat & Utilizat Fruit T, Wuhan 430064, Peoples R China
2.Univ Florida, Inst Food & Agr Sci, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA
3.Zhejiang A&F Univ, Coll Hort Sci, Hangzhou 311300, Peoples R China
关键词： Citrus; Genetic transformation Transgenic roots Gene; function
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.3； 五年影响因子：4.5 ）
ISSN： 0304-4238
年卷期： 2023 年 321 卷
页码：
收录情况： SCI
摘要： Although several methods of citrus genetic transformation have been studied and published, it is still necessary to further improve the efficiency of genetic transformation and increase the varieties used for citrus genetic transformation. Here, we report a simple, rapid and efficient root transformation technique for citrus to facilitate gene function studies. We found that infestation of Agrobacterium rhizogenes into citrus seedlings of five different species could produce stable transgenic roots in a short period after comparing and optimizing the selection of citrus species, bacterium concentration, cutting time and infection method. PCR and fluorescence observations showed a positive root transformation efficiency of approximately 13%-36% in the five tested citrus species. To demonstrate the utility of this method in the functional verification of citrus genes, a bimolecular fluorescence complementation (BiFC) assay was used to prove the interaction between CrCIPK6/9 and CrCBL5/6 in Citrus reticulata. Additionally, the PCR results showed that CrFT-RFP was transferred from the transgenic positive root to the stem in Citrus reticulata. Finally, overexpression of CjCAD from Citrus junos promoted lignin accumulation in transgenic citrus roots. This method could not only be used to study genes in roots but also various genes involved in plant growth and development. Taken together, we demonstrated that this method allows fast study of gene function in a wide range of citrus species, which would facilitate the understanding of novel genes in citrus.
- 相关文献
作者其他论文 更多>>
-
A natural mutant kumquat fruit with abnormal oil glands shows lower volatile production, better fruit flavor and higher resistance to postharvest disease
作者：Zhu, Man;Wang, Yang;Zeng, Zhebin;Wu, Xi;Teng, Jiacheng;Zhang, Jingzong;Han, Qianrong;Cheng, Yunjiang;Deng, Xiuxin;Zeng, Yunliu;Zhu, Man;Ma, Qiaoli;Chen, Chuanwu;Nieuwenhuizen, Niels J.;Atkinson, Ross G.;Xiao, Cui
关键词：Geotrichum; Limonene; Penicillium; Sensory analysis; Terpene; Transcriptomics
-
Anthocitrus: evaluation of anthocyanin accumulating "Mexican" lime fruits produced by overexpressing the Ruby transcription factor gene from Citrus sinensis "Moro"
作者：Dutt, Manjul;Mahmoud, Lamiaa M.;Weber, Kyle C.;Satpute, Aditi;Stanton, Daniel;Soares, Juliana M.;Grosser, Jude W.;Qiu, Wenming;Reuss, Laura;Wang, Yu;Killiny, Nabil
关键词：Citrus; Anthocyanins; Ruby gene; Genetic transformation; transcriptome profiling
-
Guijing2501 (Citrus unshiu) Has Stronger Cold Tolerance Due to Higher Photoprotective Capacity as Revealed by Comparative Transcriptomic and Physiological Analysis and Overexpression of Early Light-Induced Protein
作者：Xiao, Cui;He, Ligang;Qiu, Wenming;Wang, Zeqiong;He, Xiujuan;Xiao, Yuxiong;Sun, Zhonghai;Tong, Zhu;Jiang, Yingchun
关键词：citrus; cold stress; early light-induced protein; transcriptome
-
A Novel Non-Specific Lipid Transfer Protein Gene, CmnsLTP6.9, Enhanced Osmotic and Drought Tolerance by Regulating ROS Scavenging and Remodeling Lipid Profiles in Chinese Chestnut (Castanea mollissima Blume)
作者：Xiao, Yuxiong;Xiao, Cui;He, Xiujuan;Yang, Xin;Tong, Zhu;Wang, Zeqiong;Sun, Zhonghai;Qiu, Wenming
关键词：chestnut; CmnsLTP; CmnsLTP6.9; osmotic; drought
-
Overexpression of the salicylic acid binding protein 2 (SABP2) from tobacco enhances tolerance against Huanglongbing in transgenic citrus
作者：Soares, Juliana M.;Weber, Kyle C.;Mahmoud, Lamiaa M.;Grosser, Jude W.;Dutt, Manjul;Qiu, Wenming;Mahmoud, Lamiaa M.
关键词：Citrus sinensis; Huanglongbing; Transgenic; Salicylic acid; Methyl salicylate; Systemic acquired resistance
-
Evolution, gene expression, and protein-protein interaction analyses identify candidate CBL-CIPK signalling networks implicated in stress responses to cold and bacterial infection in citrus
作者：Xiao, Cui;Zhang, Hu;Xie, Fan;Qiu, Wen-Ming;Tong, Zhu;Wang, Ze-Qiong;He, Xiu-Juan;Xu, Yu-Hai;Sun, Zhong-Hai;Xie, Fan;Pan, Zhi-Yong
关键词：Citrus; CBL-CIPK interaction; Evolution; Expression pattern; Abiotic and biotic stresses
-
Insights into the mechanism of Huanglongbing tolerance in the Australian finger lime (Citrus australasica)
作者：Weber, Kyle C.;Mahmoud, Lamiaa M.;Stanton, Daniel;Welker, Stacy;Grosser, Jude W.;Levy, Amit;Dutt, Manjul;Mahmoud, Lamiaa M.;Qiu, Wenming
关键词：citrus; transcriptome; Huanglongbing; host response; pathogen-related proteins; callose deposition
