Development and validation of a PCR-based functional marker system for the brown planthopper resistance gene Bph14 in rice
文献类型： 外文期刊
作者： Zhou, Lei 1 ; Chen, Zhijun 1 ; Lang, Xuyong 1 ; Du, Bo 3 ; Liu, Kai 1 ; Yang, Guocai 1 ; Hu, Gang 1 ; Li, Sanhe 1 ; He, Guan 1 ;
作者机构： 1.Hubei Acad Agr Sci, Food Crops Inst, Hubei Key Lab Food Crop Germplasm & Genet Improve, Wuhan 430064, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
3.Wuhan Univ, Co
关键词： Oryza saliva;BPH resistance;allele-specific amplification (ASA);MAS;functional marker
期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）
ISSN： 1344-7610
年卷期： 2013 年 63 卷 3 期
页码：
收录情况： SCI
摘要： Brown planthopper (BPH) is the most damaging rice pest globally. Resistant varieties are the most effective and environmental strategy for protecting the rice crop from BPH. Functional markers (FMs) designed from polymorphic sites within gene sequences affecting phenotypic variation are highly efficient when used for marker assisted selection (MAS). Bph14 is the first and only cloned insect resistance gene so far in rice. Compared to the sequences of its non-effective alleles there are a number SNP differences. In this study, the method of allele-specific amplification (ASA) was adopted to design a simple, co-dominant, functional marker Bph14P/N for Bph14. Bph14P/N was combined with two specific dominant markers: one, named Bph14P, targets the promoter region of Bph14 and amplifies 566 bp fragments; and the other, Bph14N, targets the LRR region of bph14 and amplifies 345 bp fragments. Specificity and applicability of the functional marker system were verified in two breeding populations and a Chinese mini core collection of Oryza saliva. We recommend the use of this simple, low-cost marker system in routine genotyping for Bph14 in breeding populations.
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