One-stop polymerase chain reaction (PCR): An improved PCR method with speedy operation and comparable efficiency
文献类型： 外文期刊
作者： Bi, Yan-zhen 1 ; Zhang, Jia-nong 2 ; Ma, Jun 3 ; Ou-yang, Hui-Wu 2 ; Zheng, Xin-min 1 ; Qiao, Xian-feng 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China
2.Wuhan Univ, Coll Life Sci, RNA Grp, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
关键词： Polymerase chain reaction (PCR);post-cycling;compatible dye;Gelred
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 83 期
页码：
收录情况： SCI
摘要： The widely used polymerase chain reaction (PCR) protocol requires several post-cycling steps to visualize amplicons, decelerating PCR sample processing and result calling. "One-stop PCR" was developed by including both the loading buffer and nontoxic staining dye within a single PCR tube, allowing direct loading and simultaneous staining of amplicons for visualization. The efficiency and sensitivity of one-stop PCR were comparable to commercially available PCR kits and it is compatible with downstream uses and is not mutagenic. One-stop PCR robustly minimizes the time and labor required for PCR reaction setup and product visualization.
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