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Influences of ORF1 on the Virulence and Immunogenicity of Actinobacillus pleuropneumoniae

文献类型： 外文期刊

作者： Yuan, Fangyan 1 ; Liu, Jinlin 3 ; Guo, Yi 1 ; Tan, Chen 1 ; Fu, Shulin 1 ; Zhao, Jin 4 ; Chen, Huanchun 1 ; Bei, Weicheng 1 ;

作者机构： 1.Huazhong Agr Univ, Div Anim Infect Dis, Coll Anim Sci & Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China

3.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China

4.Cent China Normal Univ, Coll Life Sc

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN： 0343-8651

年卷期： 2011 年 63 卷 6 期

页码：

收录情况： SCI

摘要： Actinobacillus pleuropneumoniae is a Gram-negative pathogen that causes porcine pleuropneumonia. The pathogenicity of A. pleuropneumoniae is strongly correlated with the production of active repeat-in-toxin (RTX) proteins such as ApxIVA. We evaluated the contribution of a potential ApxIVA activator, ORF1, to the virulence and immunogenicity of A. pleuropneumoniae in pigs. The orf1 gene in A. pleuropneumoniae SLW03 (serovar 1, Delta apxIC Delta apxIIC) was deleted, producing strain SLW05 (Delta apxIC Delta apxIIC Delta orf1). The virulence of strains SLW03 and SLW05 was compared in pigs. Clinical signs and pulmonary lesions induced by strain SLW05 were slighter than that of strain SLW03 (P < 0.05). The immunogenicity and protective efficacy of strains SLW03 and SLW05 were similar. All pigs immunized with strain SLW03 or SLW05 developed high antibody titers against ApxIA, ApxIIA, and ApxIVA before challenge. Two weeks after a second immunization, pigs were challenged intratracheally with either a fully virulent A. pleuropneumoniae serovar 1 or serovar 3 strain. Vaccination with strains SLW03 or SLW05 provided significantly greater protection compared to the negative control (P < 0.01). Immunized pigs displayed significantly fewer clinical signs and lower lung lesion scores than non-immunized pigs. These results suggested that ORF1 plays an important role in the development of ApxIVA toxicity. Furthermore, strain SLW05 is a highly attenuated strain able to induce protective immunity against A. pleuropneumoniae infection.

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