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MicroRNA-like RNAs from the same miRNA precursors play a role in cassava chilling responses

文献类型： 外文期刊

作者： Zeng, Changying 1 ; Xia, Jing 2 ; Chen, Xin 1 ; Zhou, Yufei 1 ; Peng, Ming 1 ; Zhang, Weixiong 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Jianghan Univ, Inst Syst Biol, Wuhan 430056, Hubei, Peoples R China

3.Washington Univ, Dept Comp Sci & Engn, St Louis, MO 63130 USA

4.Washington Univ, Sch Med, Dept Genet, St Louis, MO 63130 USA

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are known to play important roles in various cellular processes and stress responses. MiRNAs can be identified by analyzing reads from high-throughput deep sequencing. The reads realigned to miRNA precursors besides canonical miRNAs were initially considered as sequencing noise and ignored from further analysis. Here we reported a small-RNA species of phased and halfphased miRNA-like RNAs different from canonical miRNAs from cassava miRNA precursors detected under four distinct chilling conditions. They can form abundant multiple small RNAs arranged along precursors in a tandem and phased or half-phased fashion. Some of these miRNA-like RNAs were experimentally confirmed by re-amplification and re-sequencing, and have a similar qRT-PCR detection ratio as their cognate canonical miRNAs. The target genes of those phased and half-phased miRNAlike RNAs function in process of cell growth metabolism and play roles in protein kinase. Half-phased miR171d. 3 was confirmed to have cleavage activities on its target gene P-glycoprotein 11, a broad substrate efflux pump across cellular membranes, which is thought to provide protection for tropical cassava during sharp temperature decease. Our results showed that the RNAs from miRNA precursors are miRNA-like small RNAs that are viable negative gene regulators and may have potential functions in cassava chilling responses.

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