Cloning and molecular characterization of a cDNA encoding a small GTPase from Hevea brasiliensis
文献类型: 外文期刊
作者: Li, H. L. 1 ; Guo, D. 1 ; Tian, W. M. 2 ; Peng, S. Q. 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Minist Agr, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Rubber, Key Lab Biol & Genet Resource Rubber Tree, Minist Agr, Danzhou, Peoples R China
关键词: GTPase;Hevea brasiliensis;Latex;Jasmonate
期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )
ISSN: 1676-5680
年卷期: 2013 年 12 卷 3 期
页码:
收录情况: SCI
摘要: Small GTPases play a critical role in the regulation of a range of cellular processes including growth, differentiation, and intracellular transportation. The cDNA encoding a small GTPase, designated as HbGTPase1, was isolated from Hevea brasiliensis. HbGTPase1 was 882 bp long containing a 612-bp open reading frame encoding a putative protein of 203 amino acids, flanked by an 83-bp 5'-untranslated region (UTR) and a 187-bp 3'-UTR. The predicted molecular mass of HbGTPase1 is 22.62 kDa, with an isoelectric point of 5.06. The HbGTPase1 protein was predicted to possess the conserved functional regions of the small GTPase superfamily of proteins. Quantitative polymerase chain reaction analysis revealed that HbGTPase1 was constitutively expressed in all tissues tested. HbGTPase1 transcripts accumulated at relatively low levels in the flower, latex, and leaves, while HbGTPase1 transcripts accumulated at relatively high levels in bark. Transcription of HbGTPase1 in the latex was induced by jasmonate.
- 相关文献
作者其他论文 更多>>
-
Selection and validation of reference genes for RT-qPCR analysis in the pericarp of Litchi chinensis
作者:Li, F.;Sun, J. H.;Men, J. L.;Li, H. L.;Wang, G.;Wang, S. J.;Wang, J. B.;Li, F.;Sun, J. H.;Men, J. L.;Li, H. L.;Wang, G.;Wang, S. J.;Wang, J. B.
关键词:Litchi chinensis Sonn; pericarp; reference genes; RT-qPCR; gene validation
-
First Report of Fusarium incarnatum Causing Fruit Rot of Litchi in China
作者:Gao, Z. Y.;Wang, J. B.;Li, M.;Gong, D. Q.;Hong, X. Y.;Li, H. L.;Hu, M. J.;Zhang, Z. K.;Wang, Y.;Zhao, C.
关键词:Fusarium incarnatum; litchi; fruit rot
-
Physiological Responses of Two Rubber Tree Clones with Differential Cold-Tolerant Potential to Cold Stress
作者:Wang, J. X.;Li, Y.;Tian, W. M.;Wang, J. X.
关键词:Hevea brasiliensis Muell. Arg.; cold stress; endogenous hydrogen peroxide; ROS scavenger; cold-tolerant rubber tree clone; cold-sensitive rubber tree clone
-
Cassava (Manihot esculenta Krantz) genome harbors KNOX genes differentially expressed during storage root development
作者:Guo, D.;Li, H. L.;Tang, X.;Peng, S. Q.;Tang, X.
关键词:KNOX transcription factor;Manihot esculenta;Differential expression;Storage root
-
Transgenic Pinus armandii plants containing BT obtained via electroporation of seed-derived embryos
作者:Liu, X. Z.;Lou, R. H.;Zhang, Y. J.;Zhang, H. Y.;Li, H. L.
关键词:Electrotransformation;insect-resistant gene;mature embryo;DNA hybridisation;Pinus armandii
-
A RAMP marker linked to the tobacco black shank resistant gene
作者:Liu, X. Z.;Yang, Y. M.;Zhang, H. Y.;He, C. S.;Li, H. L.
关键词:Tobacco;black shank resistant gene;RAMP;molecular marker
-
Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture
作者:Zhang, A. L.;Zhang, T. Y.;Luo, J. X.;Chen, S. C.;Guan, W. J.;Fu, C. Y.;Peng, S. Q.;Li, H. L.
关键词: