Transgenic Pinus armandii plants containing BT obtained via electroporation of seed-derived embryos
文献类型： 外文期刊
作者： Liu, X. Z. 1 ; Li, H. L. 2 ; Lou, R. H. 1 ; Zhang, Y. J. 1 ; Zhang, H. Y. 1 ;
作者机构： 1.SW Forestry Univ, Key Lab Forest Resources Conservat & Use SW Mt Ch, Minist Educ, Kunming 650224, Yunnan Province, Peoples R China
2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Dangzhao 571737, Hainan, Peoples R China
关键词： Electrotransformation;insect-resistant gene;mature embryo;DNA hybridisation;Pinus armandii
期刊名称：SCIENTIFIC RESEARCH AND ESSAYS （ 影响因子：0.445； 五年影响因子：0.518 ）
ISSN： 1992-2248
年卷期： 2010 年 5 卷 22 期
页码：
收录情况： SCI
摘要： A genetic transformation system for Pinus armandii was presented using electroporation for gene delivery and mature embryo as the gene target. Plasmid DNA (pBSbtCryIII(A)), which contained a selectable npt//gene for resistance to the kanamycin and a synthetic chimeric gene SbtCryIII(A) encoding the insecticidal protein btCryIII(A), was delivered into mature P. armandii embryos via electroporation. Transformed plants were identified by their ability to grow on a selective medium containing 50 mg/L of kanamycin. Plant resistance to the application of kanamycin, PCR and southern hybridization indicated that SbtCryIII(A) genes had integrated into the P. armandii genome. Results of Southern blot hybridization indicated that some PCR positive plants might be inlaid.
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