文献类型： 外文期刊
作者： Liu, X. Z. 1 ; Yang, Y. M. 1 ; He, C. S. 2 ; Li, H. L. 3 ; Zhang, H. Y. 1 ;
作者机构： 1.SW Forestry Coll, Biotechnol Lab, Kunming 650224, Yunnan Province, Peoples R China
2.S Ctr Tobacco Breeding Res China, Yuxi 653100, Yunnan, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Dangzhao 571737, Hainan, Peoples R China
关键词： Tobacco;black shank resistant gene;RAMP;molecular marker
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2009 年 8 卷 10 期
页码：
收录情况： SCI
摘要： Bulk segregant analysis (BSA) and randomly amplified microsatellite polymorphism (RAMP) were employed to analyze F(2) individuals of the Yunyan 317xHubei 517 to screen and characterize molecular markers linked to black shank resistant gene. A total of 800 arbitrary decamer oligonucleotide primer-pairs were used for RAMP analysis. Primer pair GT (CA) (4)/S89, producing one RAMP marker GT (CA) (4)/S89(550), was tightly linked to the black shank resistant gene. Results of Southern blot suggest that the fragment GT (CA) (4)/S89(550) was existed in Yunyan 317 and resistant plants, and absent in Hubei 517. Linkage analysis was carried out using marker GT (CA) (4)/S89(550) on 752 black shank high-resistant individuals of F(2) progenies from crossing between Yunyan 317 and Hubei 517. Our results indicated that the genetic distances between GT (CA) (4)/S89(550) and black shank resistant gene was 1.4cM.
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