Genetic transformation and regeneration of Hevea brasiliensis transgenic plant with GAI gene by microparticle bombardment
文献类型: 外文期刊
作者: Wang, Ying 1 ; Chen, Xiongting 1 ; Peng, Shiqing 1 ; Wu, Kunxin 1 ; Hong, Lei 2 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Lujiang High Sch Anhui Prov, Lujiang, Peoples R China
关键词: Genetic transformation;Hevea brasiliensis;Microparticle bombardment;GAI gene
期刊名称:ROMANIAN BIOTECHNOLOGICAL LETTERS ( 影响因子:0.765; 五年影响因子:0.823 )
ISSN: 1224-5984
年卷期: 2013 年 18 卷 1 期
页码:
收录情况: SCI
摘要: A typhoon is a major natural disaster in the cultivation areas of Hevea brasiliensis in China. In this study, we report a method for the transformation and regeneration of H. brasiliensis transgenic plants expressing the Arabidopsis gibberellic acid insensitive (GAI) gene to induce the dwarfing phenotype. A pBI121vector comprising CaMV35S promoter, kanamycin resistance gene (nptII), beta-glucuronidase (GUS) reporter gene, and GAI gene was used. The embryoid induction rate was 3.21% using a 40-day-old calli as target with 1100 psi helium pressure and 9 cm target distance. Kanamycin-selected calli and embryos were positive for GUS activity in histochemical assays. A transgenic line and 21 sub-plantlets were positive for GAI gene amplification using the polymerase chain reaction. The integration of the GAI gene was confirmed by Southern blot and showed a single copy insertion. These results illustrate the advantages of using microparticle bombardment for stable plant transformation in H. brasiliensis with possible applications in other plant species.
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