Genetic transformation and regeneration of Hevea brasiliensis transgenic plant with GAI gene by microparticle bombardment
文献类型: 外文期刊
作者: Wang, Ying 1 ; Chen, Xiongting 1 ; Peng, Shiqing 1 ; Wu, Kunxin 1 ; Hong, Lei 2 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Lujiang High Sch Anhui Prov, Lujiang, Peoples R China
关键词: Genetic transformation;Hevea brasiliensis;Microparticle bombardment;GAI gene
期刊名称:ROMANIAN BIOTECHNOLOGICAL LETTERS ( 影响因子:0.765; 五年影响因子:0.823 )
ISSN: 1224-5984
年卷期: 2013 年 18 卷 1 期
页码:
收录情况: SCI
摘要: A typhoon is a major natural disaster in the cultivation areas of Hevea brasiliensis in China. In this study, we report a method for the transformation and regeneration of H. brasiliensis transgenic plants expressing the Arabidopsis gibberellic acid insensitive (GAI) gene to induce the dwarfing phenotype. A pBI121vector comprising CaMV35S promoter, kanamycin resistance gene (nptII), beta-glucuronidase (GUS) reporter gene, and GAI gene was used. The embryoid induction rate was 3.21% using a 40-day-old calli as target with 1100 psi helium pressure and 9 cm target distance. Kanamycin-selected calli and embryos were positive for GUS activity in histochemical assays. A transgenic line and 21 sub-plantlets were positive for GAI gene amplification using the polymerase chain reaction. The integration of the GAI gene was confirmed by Southern blot and showed a single copy insertion. These results illustrate the advantages of using microparticle bombardment for stable plant transformation in H. brasiliensis with possible applications in other plant species.
- 相关文献
作者其他论文 更多>>
-
Capsid protein of turnip crinkle virus suppresses antiviral RNA decay by degrading Arabidopsis Dcp1 via the ubiquitination pathway (vol 121, e70075, 2025)
作者:Wu, Kunxin;Xie, Qiuxian;Liu, Xueting;Fu, Yan;Li, Shuxia;Yu, Xiaoling;Li, Wenbin;Zhao, Pingjuan;Ren, Yanli;Ruan, Mengbin;Zhang, Xiuchun;Wu, Kunxin;Xie, Qiuxian;Liu, Xueting;Fu, Yan;Li, Shuxia;Yu, Xiaoling;Li, Wenbin;Zhao, Pingjuan;Ren, Yanli;Ruan, Mengbin;Zhang, Xiuchun;Xie, Qiuxian;Liu, Xueting;Fu, Yan;Ren, Yanli;Li, Shuxia;Yu, Xiaoling;Li, Wenbin;Zhao, Pingjuan;Ruan, Mengbin;Zhang, Xiuchun
关键词:
-
Comprehensive investigation of the C2H2-type zinc finger protein transcription factor family in Aquilaria sinensis reveals the involvement of AsZFP9 in sesquiterpene biosynthesis
作者:Li, Yuji;Guo, Dong;Wang, Hao;Wang, Ying;Peng, Shiqing;Dai, Haofu;Mei, Wenli;Zhu, Jiahong;Guo, Dong;Dai, Haofu;Zhu, Jiahong;Li, Yuji;Wang, Hao;Peng, Shiqing;Dai, Haofu;Mei, Wenli
关键词:Aquilaria sinensis; Zinc finger protein; Agarwood; Sesquiterpene biosynthesis
-
Hierarchical hybrid of tungsten nitride nanosheets integrated with nitrogen-doped carbon for efficiently electrocatalytical nitrogen fixation
作者:Wang, Jing;Hui, Liufu;Chen, Jing;Chen, Milin;Huang, Ziyi;Ma, Lin;Wang, Xian;Xi, Yanjie;Gao, Yihan;Wang, Ying;Zhang, Liying;Chen, Jing;Wang, Xian
关键词:Tungsten nitride; Hierarchical structure; Nitrogen-doped carbon; Nitrogen reduction reaction; Electrocatalyst
-
Potential influence of supplemental nutrients intake by adults on the development, fecundity, and population growth of Megalurothrips usitatus (Thysanoptera: Thripidae) offspring
作者:Li, Tiantian;Liu, Ying;Liang, Xiao;Wu, Chunling;An, Xingkui;Wang, Ying;Hao, Guifeng;Chen, Yiting;Chen, Qing;Liu, Ying;Liang, Xiao;Wu, Chunling;An, Xingkui;Wang, Ying;Hao, Guifeng;Chen, Yiting;Chen, Qing;Liu, Ying;Chen, Qing
关键词:population dynamics; nutritional ecology; leguminous crops; rearing method
-
SsSGP1 peptide regulates chlorophyll degradation in sugarcane through ethylene and peroxidase pathways
作者:Wu, Kunxin;Zuang, Hongwei;Wang, Honglin;Nuo, Jia;Huang, Fuqiang;Shi, Tiantian;Liao, Wenbin;Liao, Wenbin;Wu, Kunxin;Zuang, Hongwei;Wang, Honglin;Nuo, Jia;Huang, Fuqiang;Shi, Tiantian;Liao, Wenbin
关键词:Sugarcane; Peptide; Chlorophyll; Ethylene
-
Capsid protein of turnip crinkle virus suppresses antiviral RNA decay by degrading Arabidopsis Dcp1 via ubiquitination pathway
作者:Wu, Kunxin;Xie, Qiuxian;Liu, Xueting;Fu, Yan;Li, Shuxia;Yu, Xiaoling;Li, Wenbin;Zhao, Pingjuan;Ruan, Mengbin;Zhang, Xiuchun;Wu, Kunxin;Li, Shuxia;Yu, Xiaoling;Li, Wenbin;Zhao, Pingjuan;Ruan, Mengbin;Zhang, Xiuchun;Xie, Qiuxian;Liu, Xueting;Fu, Yan;Ren, Yanli
关键词:turnip crinkle virus; viral suppressor of RNA decay; antiviral; decapping 1; protein degradation
-
Genome-wide identification of HbVQ proteins and their interaction with HbWRKY14 to regulate the expression of HbSRPP in Hevea brasiliensis
作者:Zhu, Jiahong;Qu, Long;Wang, Ying;Li, Huiliang;Peng, Shiqing;Guo, Dong;Qu, Long;Zeng, Liwang;Zhu, Jiahong;Li, Huiliang;Guo, Dong
关键词:Hevea brasiliensis; VQ protein; WRKY transcription factor; Small rubber particle protein



