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The mRNA abundance of pepc2 gene is negatively correlated with oil content in Chlamydomonas reinhardtii

文献类型： 外文期刊

作者： Deng, Xiaodong 1 ; Li, Yajun 1 ; Fei, Xiaowen 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Trop Crop Biotechnol, Minist Agr, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

2.Hainan Med Coll, Dept Biochem, Haikou 571101, Peoples R China

关键词： Phosphoenolpyruvate carboxylase gene;Oil accumulation;RNAi;Chlamydomonas reinhardtii;Nitrogen deprivation

期刊名称：BIOMASS & BIOENERGY （ 影响因子：5.061； 五年影响因子：4.815 ）

ISSN： 0961-9534

年卷期： 2011 年 35 卷 5 期

页码：

收录情况： SCI

摘要： Many microalgae accumulate oils, especially under stress conditions, thus have attracted more and more attention as a potential feedstock for biodiesel production. However, little information is available for molecular mechanisms that control oil accumulation in microalgae. In this study, we investigated the accumulation of oils during nitrogen (N) deprivation in the model green algae Chlamydomonas reinhardtii. Our results indicated that oil content significantly increased after N deficiency in C. reinhardtii, which was accompanied by a drastic decrease (63-91%) in the transcript level of phosphoenolpyruvate carboxylase 2 (PEPC2), an enzyme that converts phosphoenolpyruvate (PEP) to oxaloacetate (OAA), which conjugates with acetyl-CoA to generate citric acid. Furthermore, down regulation of pepc2 gene by RNAi silencing in C. reinhardtii led to 14-28% increase in oil content. These results revealed a negative correlation between pepc2 mRNA abundance and oil content in C. reinhardtii. (C) 2011 Elsevier Ltd. All rights reserved.

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