Helper component-proteinase (HC-Pro) protein of Papaya ringspot virus interacts with papaya calreticulin
文献类型： 外文期刊
作者： Shen, Wentao 1 ; Yan, Pu 1 ; Gao, Le 1 ; Pan, Xueying 1 ; Wu, Jinyan 1 ; Zhou, Peng 1 ;
作者机构： 1.CATAS, ITBB, Minist Agr, Key Biotechnol Lab Trop Crops, Haikou 571101, Peoples R China
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）
ISSN： 1464-6722
年卷期： 2010 年 11 卷 3 期
页码：
收录情况： SCI
摘要： P>Potyviral helper component-proteinase (HC-Pro) is a multifunctional protein involved in plant-virus interactions. In this study, we constructed a Carica papaya L. plant cDNA library to investigate the host factors interacting with Papaya ringspot virus (PRSV) HC-Pro using a Sos recruitment two-hybrid system (SRS). We confirmed that the full-length papaya calreticulin, designated PaCRT (GenBank accession no. FJ913889), interacts specifically with PRSV HC-Pro in yeast, in vitro and in plant cells using SRS, in vitro protein-binding assay and bimolecular fluorescent complementation assay, respectively. SRS analysis of the interaction between three PaCRT deletion mutants and PRSV HC-Pro demonstrated that the C-domain (residues 307-422), with a high Ca2+-binding capacity, was responsible for binding to PRSV HC-Pro. In addition, quantitative real-time reverse transcriptase-polymerase chain reaction assay showed that the expression of PaCRT mRNA was significantly upregulated in the primary stage of PRSV infection, and decreased to near-basal expression levels in noninoculated (healthy) papaya plants with virus accumulation inside host cells. PaCRT is a new calcium-binding protein that interacts with potyviral HC-Pro. It is proposed that the upregulated expression of PaCRT mRNA may be an early defence-related response to PRSV infection in the host plant, and that interaction between PRSV HC-Pro and PaCRT may be involved in plant calcium signalling pathways which could interfere with virus infection or host defence.
- 相关文献
作者其他论文 更多>>
-
Rubisco small subunit (RbCS) is co-opted by potyvirids as the scaffold protein in assembling a complex for viral intercellular movement
作者：Qin, Li;Liu, Hongjun;Liu, Peilan;Jiang, Lu;Dai, Zhaoji;Cui, Hongguang;Qin, Li;Liu, Hongjun;Liu, Peilan;Jiang, Lu;Dai, Zhaoji;Cui, Hongguang;Jiang, Lu;Li, Fangfang;Cheng, Xiaofei;Shen, Wentao;Qiu, Wenping
关键词：
-
Mutating the arginine residue within the FRNK motif of telosma mosaic virus (TelMV) HC-Pro protein attenuates viral infection and confers effective protection against TelMV in passion fruit (Passiflora edulis)
作者：Wang, Linxi;Shi, Wei;Aziz, Asma;Wang, Xiaoqing;Liu, Haobin;Cui, Hongguang;Dai, Zhaoji;Shen, Wentao
关键词：telosma mosaic virus; cross protection; HC-Pro; FRNK; passion fruit; attenuated mutant
-
Diversity and structure of the root-associated bacterial microbiomes of four mangrove tree species, revealed by high-throughput sequencing
作者：Sui, Jinlei;He, Xiaowen;Yi, Guohui;Zhou, Limin;Liu, Shunqing;Chen, Qianqian;Wu, Jinyan;Xiao, Xiaohu
关键词：Amplicon sequencing; Mangrove species; Root-associated compartments; Microbial diversity; Microbiome
-
Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu)
作者：Wang, Yaodi;Wang, Linxi;Liu, Hongjun;Gou, Bei;Hu, Weiyao;Qin, Li;Cui, Hongguang;Dai, Zhaoji;Shen, Wentao;Shen, Wentao;Wang, Aiming
关键词：Areca palm; Areca catechu; Protoplast; Protoplast isolation; PEG-mediated transformation
-
G9a-targeted chaetocin induces pyroptosis of gastric cancer cells
作者：Huang, Mian-Qing;Huang, Mian-Qing;Han, Li-Fang;Tian, Shu-Hong;Tao, Gui-Lan;Zhou, Peng;Zhou, Peng
关键词：Chaetocin; Gastric cancer; Pyroptosis; G9a; GSDME
-
A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
作者：Gou, Bei;Dai, Zhaoji;Liu, Haobin;Wang, Linxi;Liu, Peilan;Ran, Minyuan;Cui, Hongguang;Qin, Li;Wang, Yuanzheng;Fang, Chuanying;Fang, Chuanying;Zhou, Tao;Shen, Wentao;Valli, Adrian A.
关键词：Potyviridae; Potyvirus; P1; HCPro; Zn finger motif; RNA silencing suppression; host fitness; telosma mosaic virus
-
A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants
作者：Yan, Pu;Tuo, Decai;Shen, Wentao;Deng, Haida;Zhou, Peng;Yan, Pu;Tuo, Decai;Shen, Wentao;Deng, Haida;Zhou, Peng;Gao, Xinzheng
关键词：nimble cloning; plant expression vector; gene function; ectopic expression; genome editing
