Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase in Antrodia cinnamomea
文献类型： 外文期刊
作者： Li, Jing 1 ; Lin, Xiong-Jie 2 ; Shao, En-Si 1 ; Lin, Zhan-Xi 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China
2.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China
3.China Natl Engn Res Ctr JUNCAO Technol, Fuzhou 350002, Peoples R China
期刊名称：2016 2ND INTERNATIONAL CONFERENCE ON CHEMICAL MATERIALS AND PROCESS (ICCMP 2016)
ISSN： 2261-236X
年卷期： 2016 年 64 卷
页码：
收录情况： SCI
摘要： Mevalonate pyrophosphate decarboxylase (MVD) plays important roles in triterpenoid biosynthesis via the mevalonate pathway. A novel MVD gene was isolated and identified in Antrodia cinnamomea (Ac-mvd). The fulllength cDNA contained an open reading frame with a length of 1,209 bp and encoded a 402-amino acid polypeptide with a molecular mass of 43.3 kDa and a theoretical pI of 8.23. As incubation time was prolonged for 28 days, the triterpenoid content in the mycelium gradually increased and reached 39.192 +/- 2.025 mg/g; the triterpenoid content of the fruiting body grew in the hay of Cinnamomum kanehirae (ACFB-CK), was 49.391 +/- 2.675 mg/g, which was significantly different from other samples (P < 0.05). qRT-PCR revealed that the highest expression levels of Ac-mvd in the mycelium of Antrodia cinnamomea were detected on the 7th day. The expression levels gradually decreased as culture time was extended from 14 days to 42 days.
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