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Genome-wide analysis of the PHB gene family in Glycine max (L.) Merr.

文献类型: 外文期刊

作者: Song, Min 1 ; Peng, Xiangyong 1 ; Du, Caifu 2 ; Lei, Lei 2 ; Zhang, Tao 2 ; Xiang, Yang 2 ;

作者机构: 1.Qufu Normal Univ, Coll Life Sci, Qufu 273165, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550008, Guizhou, Peoples R China

关键词: Glycine max (L.) Merr.;Prohibitin domain;Gene family;Microsynteny;Expression pattern

期刊名称:GENES & GENOMICS ( 影响因子:1.839; 五年影响因子:1.329 )

ISSN: 1976-9571

年卷期: 2017 年 39 卷 10 期

页码:

收录情况: SCI

摘要: Prohibitins (PHBs) have one SPFH domain in common and present in species ranging from prokaryotes to eukaryotes. Although a number of researches on PHBs were performed in different plant species, a systematic analysis of the PHB family in soybean is still remains uncharacterized. In the present study, 24 putative PHB genes have been first systemically identified in soybean. According to phylogenetic analysis, these GmPHBs could be classified into four groups. Gene structures and motif patterns showed high levels of conservation within the phylogenetic subgroups. Several members of this family have undergone purifying selection based on Ka/Ks analysis on duplicated PHB genes in soybean. We performed microsynteny analysis across four legume species based on the comparisons among the specific regions contained in PHB genes. As a result, numerous microsyntenic gene pairs among soybean, Medicago, Lotus and Phaseolus were identified. Most soybean PHB genes exhibited different expression levels in various tissues and developmental stages through expression analysis using publicly available RNA-seq datasets. The 11 GmPHB genes from III_B subgroup were examined by qPCR for their expression in two soybean cultivar after infection by Phytophthora sojae. Besides three GmPHB genes previous reported by us, here other four genes also were rapidly induced by P. sojae infection in the resistant genotype, while induction was very weak in the susceptible genotype. The comprehensive overview of the PHB gene family in soybean genome will provide useful information for further functional analysis of the PHB gene family in soybean.

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[1]Genome-scale identification of MLO domain-containing genes in soybean (Glycine max L. Merr.). Shen, Qi,Zhao, Jinming,Xiang, Yang,Shen, Qi,Du, Caifu,Xiang, Yang,Qin, Xinrong,Cao, Jinxuan. 2012

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