文献类型： 外文期刊

作者： Wu, Tong ¹ ; Choi, Yong Soo ² ; Kim, Dong Won ² ; Wei, Xiaoping ³ ; Kang, Yuxin ¹ ; Han, Bo ¹ ; Yang, Sa ¹ ; Gao, Jing ¹ ; Dai, Pingli ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing 100193, Peoples R China

2.Natl Inst Agr Sci, Rural Dev Adm, Dept Agr Biol, Wanju Gun 55365, South Korea

3.Guizhou Acad Agr Sci, Modern Agr Dev Inst, Guiyang 550006, Peoples R China

关键词： Honey bee; Varroa destructor; Chlorothalonil; Survival; Fat body

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2024 年 204 卷

页码：

收录情况： SCI

摘要： The interaction between environmental factors affecting honey bees is of growing concern due to their potential synergistic effects on bee health. Our study investigated the interactive impact of Varroa destructor and chlorothalonil on workers' survival, fat body morphology, and the expression of gene associated with detoxification, immunity, and nutrition metabolism during their adult stage. We found that both chlorothalonil and V. destructor significantly decreased workers' survival rates, with a synergistic effect observed when bees were exposed to both stressors simultaneously. Morphological analysis of fat body revealed significant alterations in trophocytes, particularly a reduction in vacuoles and granules after Day 12, coinciding with the transition of the bees from nursing to other in-hive work tasks. Gene expression analysis showed significant changes in detoxification, immunity, and nutrition metabolism over time. Detoxification genes, such as CYP9Q2, , CYP9Q3, , and GST-D1, , were downregulated in response to stressor exposure, indicating a potential impairment in detoxification processes. Immune-related genes, including defensin-1, , Dorsal-1 , and Kayak, , exhibited an initially upregulation followed by varied expression patterns, suggesting a complex immune response to stressors. Nutrition metabolism genes, such as hex 70a, , AmIlp2, , VGMC, , AmFABP, , and AmPTL, , displayed dynamic expression changes, reflecting alterations in nutrient utilization and energy metabolism in response to stressors. Overall, these findings highlight the interactive and dynamic effects of environmental stressor on honey bees, providing insights into the mechanisms underlying honey bee decline. These results emphasize the need to consider the interactions between multiple stressors in honey bee research and to develop management strategies to mitigate their adverse effects on bee populations.