文献类型： 外文期刊

作者： Zheng, Luzhe ¹ ; Wang, Zhanzhan ³ ; Zhang, Bo ² ; Yan, Lulu ² ; Wang, Pengfei ² ; Zhao, Chao ² ; Lin, Heizhao ⁴ ; Qiu, Lihua ² ; Zhou, Chuanpeng ³ ;

作者机构： 1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

2.South China Sea Fisheries Res Inst, Key Lab Fishery Resources Dev & Utilizat South Chi, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China

3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquatic Prod Proc, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China

4.Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572426, Peoples R China

5.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518121, Peoples R China

关键词： carbohydrate; intestinal digestive enzymes; liver antioxidative enzymes; intestinal microbiota; glucose metabolism; Lateolabrax maculatus

期刊名称：FISHES （ 影响因子：2.3； 五年影响因子：2.4 ）

ISSN：

年卷期： 2023 年 8 卷 9 期

页码：

收录情况： SCI

摘要： The present study was conducted to investigate the effects of high dietary carbohydrate levels on growth performance, enzyme activities, and gene expressions related to liver glucose metabolism and the intestinal microbiota of Lateolabrax maculatus juveniles. Two experimental diets with levels of carbohydrates (20% and 30%, named the NCD group and the HCD group, respectively) were designed to feed L. maculatus (initial weight 9.45 +/- 0.03 g) for 56 days. The results showed that, compared with the NCD group, the condition factor (CF) was significantly elevated in the HCD group (p < 0.05). The plasma advanced glycosylation end products (AGEs), glycated serum protein (GSP), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and glutamate aminotransferase (AST) were significantly higher in the HCD group than those in the NCD group (p < 0.05). The intestinal lipase, chymotrypsin, and alpha-amylase in the HCD group were significantly higher than those in the NCD group (p < 0.05). The liver superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and catalase (CAT) were significantly lower in the HCD group than in the NCD group (p < 0.05). The liver malondialdehyde (MDA) and hexokinase (HK) levels were significantly higher than those in the NCD group (p < 0.05). In the histopathological findings, liver cells in the HCD group appeared to have many vacuoles, and the number of lipid droplets increased. Compared with the NCD group, the relative expression of liver glucokinase (GK) and glycogen synthetase kinase-3 (GSK3 beta) genes in the HCD group was significantly increased (p < 0.05), while the relative expression of phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) genes in the HCD group was significantly reduced (p < 0.05). High-throughput 16S rRNA gene sequencing showed that high dietary carbohydrate intake changed the composition and structure of the intestinal microbiota. At the phylum level of the intestinal microbiota, high dietary carbohydrates decreased the relative abundance of Firmicutes and increased the relative abundance of Proteobacteria and Bacteroidetes. At the genus level of the intestinal microbiota, high carbohydrates decreased the relative abundance of Bacillus and increased the relative abundance of Photobacterium and Paraclostridium. From the results of this experiment on L. maculatus, high carbohydrates led to increased condition factor and liver glycogen, lipid deposition, decreased antioxidant capacity of the liver, increased relative abundance of harmful intestinal microorganisms, and disrupted glucose metabolism.