EFFECT OF SEMEN EXTENDER SUPPLEMENTATION WITH TREHALOSE, VITAMIN C AND E ON POST-THAW MIN PIG SPERM QUALITIES
文献类型: 外文期刊
作者: Ma, Hong 1 ; Liu, Di 1 ; Wang, Wentao 1 ; Wang, Liang 1 ; Fu, Bo 1 ; Li, Zhongqiu 1 ; He, Xinmiao 1 ;
作者机构: 1.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China
关键词: sperm quality;cryopreservation;min pig
期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )
ISSN: 0143-2044
年卷期: 2015 年 36 卷 5 期
页码:
收录情况: SCI
摘要: BACKGROUND: Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource. OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig. MATERIALS AND METHODS: Ejaculated mm pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated. RESULTS: The quality of post-thawed spenn samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05). CONCLUSION: Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
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