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EFFECT OF SEMEN EXTENDER SUPPLEMENTATION WITH TREHALOSE, VITAMIN C AND E ON POST-THAW MIN PIG SPERM QUALITIES

文献类型： 外文期刊

作者： Ma, Hong 1 ; Liu, Di 1 ; Wang, Wentao 1 ; Wang, Liang 1 ; Fu, Bo 1 ; Li, Zhongqiu 1 ; He, Xinmiao 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

关键词： sperm quality;cryopreservation;min pig

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2015 年 36 卷 5 期

页码：

收录情况： SCI

摘要： BACKGROUND: Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource. OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig. MATERIALS AND METHODS: Ejaculated mm pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated. RESULTS: The quality of post-thawed spenn samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05). CONCLUSION: Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.

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