Expression Profiles of the Heat Shock Protein 70 Gene in Response to Heat Stress in Agrotis c-nigrum (Lepidoptera: Noctuidae)
文献类型： 外文期刊
作者： Wang, Ling 1 ; Yang, Shuai 1 ; Zhao, Kuijun 1 ; Han, Lanlan 1 ;
作者机构： 1.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China
2.Heilongjiang Acad Agr Sci, Virus Free Seedling Res Inst, Harbin 150086, Peoples R China
关键词： heat shock protein 70;Agrotis c-nigrum Linnaeus;expression profile;heat stress
期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）
ISSN： 1536-2442
年卷期： 2015 年 15 卷
页码：
收录情况： SCI
摘要： Heat shock proteins (HSPs) are molecular chaperones, and their overexpression enhances the survivability and stress tolerance of the cell. To understand the characteristics of HSP70 in Agrotis c-nigrum Linnaeus larvae, the coding sequence of this protein was cloned, and the effect of heat stress on transcription and protein properties was assessed. The obtained cDNA sequence of HSP70 was 2,213 bp, which contained an ORF of 1,965 bp and encoded 654 amino acid residues. Isolated HSP70 cDNA demonstrated more than 80% identity with the sequences of other known insect HSP70s. Next, HSP70 was expressed in Escherichia coli BL21 (DE3) cells and identified using SDS-PAGE and western blotting analyses. In addition, anti-HSP70-specific antisera were prepared using a recombinant HSP70 protein, and the results showed that this antisera was very specific to AcHSP70. Real-time quantitative polymerase chain reaction detected the relative transcription of the HSP70 gene in larvae and the transcription of A. c-nigrum in response to high temperatures. Induction of HSP70 was up-regulated to peak expression at 36 degrees C.
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