文献类型： 外文期刊
作者： Li, Meng 1 ; Feng, Liu 2 ; Shao, Shushuang 1 ; Tao, Yang 1 ; Fu, Haiyan 1 ; Hong, Lei 1 ; Li, Xiuliang 1 ; Han, Dequan 1 ; P 1 ;
作者机构： 1.Heilongjiang Univ, Engn Res Ctr Agr Microbiol Technol, Harbin 150080, Peoples R China
2.Heilongjiang Acad Agr Sci, Safty & Qual Inst Agr Prod, Harbin 150086, Peoples R China
关键词： Kazachstania unispora;cryoprotectant;orthogonal experiment;optimization
期刊名称：2015 4TH INTERNATIONAL CONFERENCE ON ENERGY AND ENVIRONMENTAL PROTECTION (ICEEP 2015)
ISSN：
年卷期： 2015 年
页码：
收录情况： SCI
摘要： Cryoprotectants were selected and optimized to reduce the damage in freeze-drying process to Kazachstania unispora Y15 through single-factor experiment and orthogonal experiment. Evaluated by the survival rate of cells, cryoprotectants and the amount used were determined through single-factor experiment. The optimum formulation of cryoprotectant was gotten in orthogonal experiment: sucrose 22g/100mL, skim milk 15g/100mL, polyethylene glycol-1000 1.1g/100mL, L-glutamic acid sodium 7g/100mL, Kazachstania unispora Y15 freeze-dried survival rate was 94.11% with the optimum cryoprotectants.
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