您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Improved taxol production in Nodulisporium sylviforme derived from inactivated protoplast fusion

文献类型： 外文期刊

作者： Zhao, Kai 1 ; Sun, Lixin 3 ; Ma, Xi 1 ; Li, Xiuliang 1 ; Wang, Xin 1 ; Ping, Wenxiang 1 ; Zhou, Dongpo 1 ;

作者机构： 1.Heilongjiang Univ, Key Lab Microbiol, Coll Life Sci, Harbin 150080, Peoples R China

2.Heilongjiang Acad Agr Sci, Biotechnol Res Ctr, Harbin 150086, Peoples R China

3.Hosp Heilongjiang Univ, Harbin 15008, Peoples R China

关键词： Taxol;endophytic fungi;protoplast preparation;protoplast fusion

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 20 期

页码：

收录情况： SCI

摘要： Inactivated protoplast fusion by UV irradiation and UV+LiCl mutation was conducted using Nodulisporium sylviforme strain UV40-19 and UL50-6 to breed a high taxol-producing fungus. Qualitative and quantitative analysis of taxol production was confirmed using thin-layer chromatography, high performance liquid chromatography and mass spectrometry. The protoplasts of UV40-19 and UL50-6 were fully inactivated by heating at 54 degrees C for 5 min and by UV irradiation (30 w UV light and vertical distance 30 cm) for 85 s. The highest fusion rate (14.31 +/- 1.13%) between UV40-19 and UL50-6 was obtained under the conditions of 35% PEG, 90 s fusion time and the addition of 0.01 mol/l CaCl2. One high taxol production strain HDF-68 was obtained. The taxol production was up to 468.62 +/- 37.49 mu g/l, which was increased by 24.51 and 19.35% compared with the parental strain UV40-19 and UL50-6, respectively. This study provided a good basis for the application of this technique to the breeding of the strains with high taxol output.

  • 相关文献

[1]Investigation of fermentation conditions and optimization of medium for taxol production from taxol-producing fungi. Zhao, Kai,Li, Zhugang,Ge, Nan,Li, Xiuliang,Wang, Xin,Zhou, Dongpo,Zhao, Kai. 2011

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台