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Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene

文献类型: 外文期刊

作者: Juan, J. X. 1 ; Yu, X. H. 1 ; Jiang, X. M. 1 ; Gao, Z. 3 ; Zhang, Y. 4 ; Li, W. 1 ; Duan, Y. D. 5 ; Yang, G. 6 ;

作者机构: 1.Northeast Agr Univ, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, Harbin, Peoples R China

2.Collaborat Innovat Ctr Dev & Utilizat Forest Reso, Harbin, Peoples R China

3.Northeast Agr Univ, Harbin Baidu Sci & Technol Dev Co Ltd, Harbin, Peoples R China

4.Heilongjiang Acad Sci, Nat & Ecol Res Inst, Harbin, Heilongjiang, Peoples R China

5.Heilongjiang Acad Agr Sci, Berries Res Inst, Suiling, Heilongjiang, Peoples R China

6.Heilongjiang Acad Agr Sci, Berries Res Inst, Suiling, Heilon

关键词: Cold transcription factor;ICE1 gene;Tomato;Plant expression vector

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 1 期

页码:

收录情况: SCI

摘要: ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive. After low-temperature stress treatment, praline content and peroxide and catalase activities in the transgenic tomato plants were higher compared with non-transgenic controls, while malondialdehyde content was clearly lower.

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