Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala)
文献类型： 外文期刊
作者： Wang, Y. S. 1 ; Liu, Z. Y. 1 ; Li, Y. F. 3 ; Zhang, Y. 1 ; Yang, X. F. 1 ; Feng, H. 1 ;
作者机构： 1.Shenyang Agr Univ, Dept Hort, Shenyang, Peoples R China
2.Qiqihar Univ, Dept Life Sci Agr & Forestry, Qiqihar, Peoples R China
3.Heilongjiang Acad Agr Sci, Hort Sub Acad, Harbin, Peoples R China
关键词： Ornamental kale;Sequence-related amplified polymorphism;Red leaf
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2013 年 12 卷 2 期
页码：
收录情况： SCI
摘要： Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line 'D05' with a white leaf double-haploid line 'D10' was analyzed for the red leaf trait. By combining bulked segregant analysis and sequence-related amplified polymorphism technology, we identified 3 markers linked to the Re/re locus. A genetic map of the Re locus was constructed using these sequence-related amplified polymorphism markers. Two of the markers, Me8Em4 and Me8Em17, were located on one side of Re/re at distances of 2.2 and 6.4 cM, whereas the other marker, Me9Em11, was located on the other side of Re/re at a distance of 3.7 cM. These markers could be helpful for the subsequent cloning of the red trait gene and marker-assisted selection in ornamental kale breeding programs.
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