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Differentially Expressed Genes of Soybean During Infection by Phytophthora sojae

文献类型： 外文期刊

作者： Xu Peng-fei 1 ; Wu Jun-jiang 2 ; Xue, Allen 3 ; Li Wen-bin 1 ; Chen Wei-yuan 4 ; Wei Lai 2 ; Lv Hui-ying 5 ; Lin Shi-feng;

作者机构： 1.NE Agr Univ, Key Lab Soybean Biol, Minist Educ, Soybean Res Inst, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Soybean Res Inst, Harbin 150086, Peoples R China

3.Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada

4.Heilongjiang Acad Agr Sci, Suihua Res Inst, Suihua 152052, Peoples R China

5.Chinese Acad Sci, Inst

关键词： cDNA microarray;Glycine max;Phytophthora sojae;soybean;suppressed subtraction hybridization

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2012 年 11 卷 3 期

页码：

收录情况： SCI

摘要： To elucidate the differential gene expression patterns in soybeans during infection by Phytophthora sojae, a cDNA library for suppression subtractive hybridization (SSH) was constructed with cDNAs from soybean cultivar Suinong 10 treated with sterile distilled water as the driver and cDNAs from Suinong 10 inoculated with P sojae as the tester. A total of 2 067 recombinant colonies from the SSH library were randomly picked, amplified, and sequenced. After discarding 312 poor quality expressed sequence tags (EST), 1 755 high quality ESTs were assembled and edited to 1 384 tentatively unique genes (TUG), in which, 586 showed significant homology to known sequences, and 798 had low homology or no match with the known sequences. A cDNA microarray containing 307 singletons from the 586 TUGs and 222 singletons from the 798 TUGs was developed to characterize differentially expressed cDNAs in the SSH library, and eight cDNAs were identified to be up-regulated after microarray analysis and then confirmed by real-time PCR. They were homologous to the protein 10, and were also related to some proteins in disease resistance response, such as pathogen-related protein, phenylalanine ammonia-lyase, isoflavone reductase, WRKY transcription factor 31, major allergen Pru ar 1, and pleiotropic drug resistance protein 12. Most of the up-regulated cDNAs encode enzymes of phytoalexin biosynthesis and pathogenesis-related proteins involved in plant disease resistance. Here, we fist reported the Pru ar 1 in soybeans. The findings of this research have contributed to better understanding of soybean resistance to P. sojae at the molecular level.

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