Overexpression of Shinorhizobium meliloti flavohemoglobin improves cell growth and fatty acid biosynthesis in oleaginous fungus Mucor circinelloides
文献类型： 外文期刊
作者： Zhang, Huaiyuan 1 ; Kang, Xinxin 2 ; Wang, Ruixue 1 ; Xin, Feifei 1 ; Chang, Yufei 1 ; Zhang, Yingtong 3 ; Song, Yuanda 1 ;
作者机构： 1.Shandong Univ Technol, Colin Ratledge Ctr Microbial Lipids, Sch Agr Engn & Food Sci, 266 Xincun West Rd, Zibo 255000, Shandong, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Yushan Rd 5, Qingdao 266003, Shandong, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Nanjing 210014, Jiangsu, Peoples R China
关键词： Fatty acid biosynthesis; Flavohemoprotein; Hemoprotein; Mucor circinelloides
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.716； 五年影响因子：2.672 ）
ISSN： 0141-5492
年卷期： 2022 年 44 卷 4 期
页码：
收录情况： SCI
摘要： Oxygen availability is a limiting factor for lipid biosynthesis in eukaryotic microorganisms. Two bacterial hemoglobins from Vitreoscilla sp. (VHb) and Shinorhizobium meliloti (SHb), which deliver oxygen to the respiratory chain to produce more ATP, were introduced into Mucor circinelloides to alleviate oxygen limitation, thereby improving cell growth and fatty acid production. The VHb and SHb genes were integrated into the M. circinelloides MU402 genome by homologous recombination. VHb and SHb protein expression was verified by carbon monoxide difference spectrum analysis. The biomass was increased by similar to 50% in the strain expressing SHb compared with VHb. The total fatty acid (TFA) content of the strain expressing SHb reached 15.7% of the dry cell weight (similar to 40% higher than that of the control strain) during flask cultivation. The biomass and TFA content were markedly increased (12.1 g/L and 21.1% dry cell weight, respectively) in strains expressing SHb than strains expressing VHb during fermenter cultivation. VHb and SHb expression also increased the proportion of polyunsaturated fatty acids. Overexpressed bacterial hemoglobins, especially SHb, increased cell growth and TFA content in M. circinelloides at low and high aeration, suggesting that SHb improves fatty acid production more effectively than VHb in oleaginous microorganisms.
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