Resistance of Xanthomonas oryzae pv. oryzae to Lytic Phage X2 by Spontaneous Mutation of Lipopolysaccharide Synthesis-Related Glycosyltransferase
文献类型： 外文期刊
作者： Zhang, Muchen 1 ; Qian, Jiahui 1 ; Xu, Xinyan 1 ; Ahmed, Temoor 1 ; Yang, Yong 3 ; Yan, Chenqi 3 ; Elsharkawy, Mohsen Mohamed 5 ; Hassan, Mohamed M. 6 ; Alorabi, Jamal A. 6 ; Chen, Jianping 3 ; Li, Bin 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China
2.Zhejiang Univ, Inst Biotechnol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
4.Ningbo Acad Agr Sci, Inst Biotechnol, Ningbo 315040, Peoples R China
5.Kafrelsheikh Univ, Fac Agr, Agr Bot Dept, Kafr Al Sheikh 33516, Egypt
6.Taif Univ, Coll Sci, Dept Biol, POB 21974, Taif, Saudi Arabia
7.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
关键词： Xanthomonas oryzae pv; oryzae; phage resistance; virulence; lipopolysaccharide; glycosyltransferase
期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）
ISSN：
年卷期： 2022 年 14 卷 5 期
页码：
收录情况： SCI
摘要： Phage therapy is a promising biocontrol management on plant diseases caused by bacterial pathogens due to its specificity, efficiency and environmental friendliness. The emergence of natural phage-resistant bacteria hinders the application of phage therapy. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of the devastating bacterial leaf blight disease of rice. Here, we obtained a spontaneous mutant C2R of an Xoo strain C2 showing strong resistance to the lytic phage X2. Analysis of the C2R genome found that the CDS2289 gene encoding glycosyltransferase acquired a frameshift mutation at the 180th nucleotide site, which also leads to a premature stop mutation at the 142nd amino acid. This mutation confers the inhibition of phage adsorption through the changes in lipopolysaccharide production and structure and bacterial surface morphology. Interestingly, glycosyltransferase-deficient C2R and an insertional mutant k2289 also showed reduced virulence, suggesting the trade-off costs of phage resistance. In summary, this study highlights the role of glycosyltransferase in interactions among pathogenic bacteria, phages and plant hosts, which provide insights into balanced coevolution from environmental perspectives.
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