TrpR-Like Protein PXO_00831, Regulated by the Sigma Factor RpoD, Is Involved in Motility, Oxidative Stress Tolerance, and Virulence in Xanthomonas oryzae pv. oryzae
文献类型： 外文期刊
作者： Xu, Zhizhou 1 ; Wu, Guichun 2 ; Wang, Bo 2 ; Zhao, Yancun 2 ; Liu, Fengquan 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Jiangsu, Peoples R China
3.Anhui Agr Univ, Sch Life Sci, Natl Engn Lab Crop Stress Resistance Breeding, Hefei 230036, Anhui, Peoples R China
关键词： Oryza sativa; pathogenicity; transcription; tryptophan repressor; Xanthomonas oryzae pv; oryzae
期刊名称：PHYTOPATHOLOGY （ 影响因子：3.2； 五年影响因子：3.9 ）
ISSN： 0031-949X
年卷期： 2023 年 113 卷 2 期
页码：
收录情况： SCI
摘要： Xanthomonas oryzae pv. oryzae (Xoo) is a Gram-negative bacterium that causes bacterial leaf blight in rice. In this study, we identified a putative TrpR-like protein, PXO_TrpR (PXO_00831), in Xoo. This protein contains a tryptophan (Trp) repressor domain and is highly conserved in Xanthomonas. Auxotrophic assays and RT-qPCR confirmed that PXO_TrpR acts as a Trp repressor, negatively regulating the expression of Trp biosynthesis genes. Pathogenicity tests showed that PXO_trpR knockout in Xoo significantly reduced lesion development and disease symptoms in the leaves of susceptible rice. RNA-seq analysis and phenotypic tests revealed that the PXO_trpR mutant exhibited impaired cell motility and was more sensitive to H2O2 oxidative stress than the wild-type strain. Furthermore, we found that the sigma 70 factor RpoD controlled the transcription of PXO_trpR by directly binding to its promoter region. This study demonstrates the biological function and transcriptional mechanism of PXO_TrpR as a Trp repressor in Xoo and evaluates its novel pathogenic roles by regulating flagellar motility and the oxidative stress response.
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