MiR-26a Inhibits Porcine Adipogenesis by Regulating ACADM and ACSL1 Genes and Cell Cycle Progression
文献类型： 外文期刊
作者： Zhang, Dongjie 1 ; Hao, Wanjun 2 ; Zhu, Rongru 2 ; Wang, Liang 1 ; Wu, Xiaoxu 2 ; Tian, Ming 1 ; Liu, Di 1 ; Yang, Xiuqin 2 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin 150086, Peoples R China
2.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China
关键词： pig; adipogenesis; miR-26a; ACADM; ACSL1; RNA-seq
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2024 年 14 卷 23 期
页码：
收录情况： SCI
摘要： MicroRNAs play essential roles in biological processes by regulating gene expression at the post-transcriptional level. Our previous studies suggested the role of miR-26a in porcine fat accumulation. Here, through gain- and loss-of-function analyses, we first showed that miR-26a increased the proliferation of porcine preadipocytes by promoting cell division and that miR-26a inhibited the preadipocyte differentiation. Next, acyl-CoA dehydrogenase, medium chain (ACADM) was revealed to promote the proliferation and differentiation of preadipocytes for the first time. Then, it was revealed that miR-26a regulates adipogenesis by directly binding to the 3 ' untranslated region of ACADM and the long-chain acyl-Co A synthetase 1 (ACSL1) gene, a previously known regulator of adipogenesis. Finally, RNA-sequencing, performed on preadipocytes overexpressing miR-26a, identified 337 differentially expressed genes in the early stage of adipogenesis; among them, nine genes were characterized as potential targets of miR-26a. The 337 genes were mainly involved in Gene Ontology terms related to cell division, indicating that cell cycle progression was also a major event regulated by miR-26a during adipogenesis. We provide novel data for understanding the molecular mechanisms underlying adipogenesis, which will contribute to controlling fat accumulation in animals.
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