文献类型： 外文期刊

作者： Cai, Hanyang ¹ ; Huang, Youmei ¹ ; Liu, Liping ² ; Zhang, Man ¹ ; Chai, Mengnan ¹ ; Xi, Xinpeng ¹ ; Aslam, Mohammad ¹ ; Wang, Lulu ⁴ ; Ma, Suzhuo ¹ ; Su, Han ¹ ; Liu, Kaichuang ¹ ; Tian, Yaru ¹ ; Zhu, Wenhui ¹ ; Qi, Jingang ¹ ; Dresselhaus, Thomas ² ; Qin, Yuan ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou 350002, Peoples R China

2.Univ Regensburg, Cell Biol & Plant Biochem, D-93053 Regensburg, Germany

3.Guangdong Acad Agr Sci, Tea Res Inst, Guangdong Prov Key Lab Tea Plant Resources Innovat, Dafeng Rd 6, Guangzhou 510640, Peoples R China

4.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangxi Key Lab Sugarcane Biol, Nanning 530004, Peoples R China

期刊名称：PLANT CELL （ 影响因子：11.6； 五年影响因子：12.9 ）

ISSN： 1040-4651

年卷期： 2023 年 35 卷 5 期

页码：

收录情况： SCI

摘要： In most flowering plants, the female germline is initiated in the subepidermal L2 layer of ovule primordia forming a single megaspore mother cell (MMC). How signaling from the L1 (epidermal) layer could contribute to the gene regulatory network (GRN) restricting MMC formation to a single cell is unclear. We show that EPIDERMAL PATTERNING FACTOR-like (EPFL) peptide ligands are expressed in the L1 layer, together with their ERECTA family (ERf) receptor kinases, to control female germline specification in Arabidopsis thaliana. EPFL-ERf dependent signaling restricts multiple subepidermal cells from acquiring MMC-like cell identity by activating the expression of the major brassinosteroid (BR) receptor kinase BRASSINOSTEROID INSENSITIVE 1 and the BR-responsive transcription factor BRASSINOZOLE RESISTANT 1 (BZR1). Additionally, BZR1 coordinates female germline specification by directly activating the expression of a nucleolar GTP-binding protein, NUCLEOSTEMIN-LIKE 1 (NSN1), which is expressed in early-stage ovules excluding the MMC. Mutants defective in this GRN form multiple MMCs resulting in a strong reduction of seed set. In conclusion, we uncovered a ligand/receptor-like kinase-mediated signaling pathway acting upstream and coordinating BR signaling via NSN1 to restrict MMC differentiation to a single subepidermal cell. A ligand/receptor-like kinase-mediated signaling pathway coordinates brassinosteroid signaling via the GTP-binding protein NUCLEOSTEMIN-LIKE 1 to restrict megaspore mother cell differentiation to a single subepidermal cell.