Colonization Mechanism of Endophytic Enterobacter cloacae TMX-6 on Rice Seedlings Mediated by Organic Acids Exudated from Roots
文献类型: 外文期刊
作者: Wang, Pei 1 ; Ma, Liya 2 ; Ge, Jing 2 ; Feng, Fayun 2 ; Wan, Qun 2 ; Zeng, Dongqiang 1 ; Yu, Xiangyang 1 ;
作者机构: 1.Guangxi Univ, Coll Agr, Natl Demonstrat Ctr Expt Plant Sci Educ, Guangxi Key Lab Agrioenvironm & Agr Prod Safety, Nanning 530004, Guangxi, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, Jiangsu, Peoples R China
关键词: root exudates; rhizosphere directional regulation; thiamethoxam; chemotaxis; biofilm formation
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:6.1; 五年影响因子:6.3 )
ISSN: 0021-8561
年卷期: 2023 年 71 卷 12 期
页码:
收录情况: SCI
摘要: Small molecular organic acids (SMOAs) in root exudates are critical for plant-microbe interaction, especially under environmental stresses. However, the dominant organic acids driving the process and promoting the colonization are unclear. Here, using a target metabolomics, 20 main SMOAs of rice root exudates were identified and analyzed in control and 10 mg/L thiamethoxam-treated groups. The composition of these SMOAs differed significantly between the two treatments. Among which, malic acid, citric acid, succinic acid, and proline induced a chemotactic response, swimming ability, and biofilm formation of Enterobacter cloacae TMX-6 in a dose-dependent manner. The maximal chemotactic response of TMX-6 was induced by proline at 10 mg/L, and a strong chemotactic response was even observed at 0.01 mg/L. The recruitment assay confirmed that the addition of these four compounds promoted the colonization of TMX-6. The results provide insight for directional regulation of plant-microbe interactions for beneficial outcomes.
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