Isolation, antioxidant property and protective effect on PC12 cell of the main anthocyanin in fruit of Lycium ruthenicum Murray
文献类型： 外文期刊
作者： Tang, Jilong 1 ; Yan, Yamei 1 ; Ran, Linwu 3 ; Mi, Jia 2 ; Sun, Yi 1 ; Lu, Lu 2 ; Gao, Yuxiao 1 ; Zeng, Xiaoxiong 1 ; Cao, Youlong 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
2.Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Peoples R China
3.Ningxia Med Univ, Lab Anim Ctr, Yinchuan 750004, Peoples R China
关键词： Lycium ruthenicum; Anthocyanin; Antioxidant property; PC12 cell; Cytoprotective effect
期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：5.223； 五年影响因子：5.178 ）
ISSN： 1756-4646
年卷期： 2017 年 30 卷
页码：
收录情况： SCI
摘要： The fruit of Lycium ruthenicum Murray has been widely used as a precious Chinese herbal medicine. In this study, the major anthocyanin in L ruthenicum fruit was obtained via an AKTA purifier system with YMC-Pack ODS-A column and identified by mass and nuclear magnetic resonance spectroscopy to be petunidin 3-0-[6-0-(4-0-(trans-p-coumaroyl)-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside]-5-0-[beta-D-glucopyranoside] (compound 1). Compound 1 exhibited higher scavenging activities against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), 1,1-dipheny1-2-picrylhydra-zyl and superoxide radicals than the crude extract of anthocyanins. Moreover, compound 1 was demonstrated to have potential cyto-protective effect on hydrogen peroxide-induced intracellular reactive oxygen damage in neuronlike rat pheochromocytoma line cells by promoting cell proliferation, mitigating lipid peroxidation and regulating endogenous antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase). (C) 2017 Elsevier Ltd. All rights reserved.
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