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Function analysis of the cytidine deaminases in root-knot nematode, Meloidogyne incognita

文献类型： 外文期刊

作者： Wang, Dongwei 1 ; Wang, Jian 2 ; Li, Yingmei 3 ; Zhang, Zhuo 1 ; Cheng, Jue 1 ; Zhu, Chunhui 1 ; Zhao, Zhixiang 4 ; Tan, Xinqiu 1 ; Zhang, Deyong 1 ; Liu, Yong 1 ; Cheng, Feixue 1 ;

作者机构： 1.Hunan Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Management Pests & Dis Hort Cro, Changsha 410125, Peoples R China

2.Hunan Acad Agr Sci, Agr Econ & Reg Planning Res Inst, Changsha 410125, Peoples R China

3.Bioagr Inst Shanxi, Shaanxi Key Lab Plant Nematol, Xian 710043, Peoples R China

4.Hainan Acad Agr Sci, Inst Plant Protect, Res Ctr Qual Safety & Stand Agro Prod, Haikou 571100, Peoples R China

5.Key Lab Plant Dis & Pest Control Hainan Prov, Haikou 571100, Peoples R China

关键词： Root-knot nematodes; Cytidine deaminases; RNA interference; Development; Pathogenesis

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.9； 五年影响因子：2.0 ）

ISSN： 0929-1873

年卷期： 2025 年 172 卷 1 期

页码：

收录情况： SCI

摘要： Meloidogyne incognita is one of the most widespread and destructive plant pathogenic nematodes in the world. Cytidine deaminase proteins (CDDs) belong to a family of enzymes that catalyse the deamination of cytidine to uridine in bacteria and fungi, and have an important role in the pyrimidine salvage pathway. A cDNA with homology to the CDDs family of M. incognita was identified in this study. We obtained the full-length sequence of Mi-CDD1 from M. incognita, which is 595 bp long and includes a 456 bp ORF that encodes 151 amino acids. Multiple sequence alignment showed that Mi-CDD1 contains a conservative protein kinase C (PKC) phosphorylation site, the phylogenetic tree indicated that Mi-CDD1 had the closest relationship with M. graminicola CDD. Mi-CDD1 mRNA was present in the intestine of M. incognita and targeted in the cytoplasm. The relative expression of Mi-CDD1 was significantly highest in eggs than the other development stages, than in second-stage juveniles. The relative expression of Mi-CDD1 was lowest after soaking with Mi-CDD1 dsRNA in vitro for 72 h than the other treatments using in vitro RNAi. The pathogenicity of M. incognita to pepper was significantly reduced after treatment with Mi-CDD1 dsRNA in vitro for 72 h, but the hatching rate of M. incognita eggs after soaking with Mi-CDD1 dsRNA was not significantly different among Mi-CDD1, GFP dsRNA in vitro, and control treatments. The results help to understand the function of Mi-CDD1 in M. incognita.

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