您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of Acinetobacter schindleri isolated from Chinese giant salamanders ( Andrias davidianus )

文献类型： 外文期刊

作者： Wang, Cheng 1 ; Xie, Yixing 2 ; Deng, Zhiyong 2 ; Yuan, Huanyan 2 ; Tian, Mingzhu 2 ; Mao, Pan 2 ; Zhou, Yong 3 ; Wei, Ying 2 ;

作者机构： 1.Hunan Zhangjiajie Giant Salamander Natl Nat Reserv, Zhangjiajie 427400, Peoples R China

2.Hunan Zhangjiajie Giant Salamander Natl Nat Reserv, Zhangjiajie, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China

关键词： Andrias davidianus; Acinetobacter schindleri; 16S rRNA gene sequencing; histopathology

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.5； 五年影响因子：0.6 ）

ISSN： 0792-156X

年卷期： 2024 年 76 卷 2 期

页码：

收录情况： SCI

摘要： At a particular aquaculture facility in Zhangjiajie in China, the Chinese giant salamander ( Andrias davidianus ) exhibited analogous clinical manifestations, culminating in sequential mortalities. This study used rigorous aseptic sampling methods to isolate bacteria from the affected liver of salamanders to determine the causative agent behind the decline in amphibians. A bacterial pathogen was isolated from diseased A. davidianus , and the strain was named DN-3. This isolate was subjected to bacterial identification, antibiotic susceptibility assays, reinfection experiments, and biochemical profiling. The isolated bacterial strain was definitively identified as Acinetobacter schindleri using 16S rRNA sequence analysis and biochemical identification. Antibiotic susceptibility testing revealed that this isolate was susceptible to neomycin, macrolides, doxycycline, piperacillin, nitrofurantoin, and carbenicillin. Subsequent reinfection assays, in which varying concentrations of the bacterial inoculum were administered to healthy salamanders, confirmed that the pathogen elicited varying degrees of morbidity or mortality within an eight -day observation period. The median lethal dose (LD50) of A. schindleri DN-3 for A. davidianus was calculated to be 6.25x104 CFU/mL. This result supports the significant pathogenicity of the strain for A. davidianus . The findings of this study provide empirical insights into the clinical management and epidemiological control of diseases affecting A. davidianus .

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台