Transcriptome analysis reveals a new virulence-associated trimeric autotransporter responsible for Glaesserella parasuis autoagglutination
文献类型： 外文期刊
作者： Li, Junxing 1 ; Ye, Shiyi 1 ; Su, Fei 1 ; Yu, Bin 1 ; Xu, Lihua 1 ; Sun, Hongchao 1 ; Yuan, Xiufang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310021, Peoples R China
关键词： Glaesserella parasuis; transcriptome analysis; autoagglutination; Wza; vtaA31
期刊名称：VETERINARY RESEARCH （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN： 0928-4249
年卷期： 2024 年 55 卷 1 期
页码：
收录情况： SCI
摘要： Capsular polysaccharide is an important virulence factor of Glaesserella parasuis. An acapsular mutant displays multiple phenotype variations, while the underlying mechanism for these variations is unknown. In this study, we created an acapsular mutant by deleting the wza gene in the capsule locus. We then used transcriptome analysis to compare the gene expression profiles of the wza deletion mutant with those of the parental strain to understand the possible reasons for the phenotypic differences. The mutant Delta wza, which has a deleted wza gene, secreted less polysaccharide and lost its capsule structure. The Delta wza exhibited increased autoagglutination, biofilm formation and adherence to eukaryotic cells, while the complementary strain C-Delta wza partially restored the phenotype. Transcriptome analysis revealed several differentially expressed genes (DEGs) in Delta wza, including up-regulated outer membrane proteins and proteins involved in peptidoglycan biosynthesis, suggesting that wza deletion affects the cell wall homeostasis of G. parasuis. Transcriptome analysis revealed the contribution of non-coding RNAs in the regulation of DEGs. Moreover, a new virulence-associated trimeric autotransporter, VtaA31 is upregulated in Delta wza. It is responsible for enhanced autoagglutination but not for enhanced biofilm formation and adherence to eukaryotic cells in Delta wza. In conclusion, these data indicate that wza affects the expression of multiple genes, especially those related to cell wall synthesis. Furthermore, they provide evidence that vtaA31 is involved in the autoagglutination of G. parasuis.
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