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Linkage and association mapping and Kompetitive allele-specific PCR marker development for improving grain protein content in wheat

文献类型： 外文期刊

作者： Jiang, Peng 1 ; Zhang, Peng 1 ; Wu, Lei 1 ; He, Yi 1 ; Li, Chang 1 ; Ma, Hongxiang 1 ; Zhang, Xu 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Jiangsu Key Lab Crop Genome & Mol Breeding, Yangzhou 225009, Jiangsu, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2021 年 134 卷 11 期

页码：

收录情况： SCI

摘要： Key Message Linkage and association mapping identified nine candidate intervals for wheat GPC, and large-scale association mapping based on 9 corresponding KASP markers and 1163 F-4 breeding lines revealed 3 significant markers. Wheat grain protein content (GPC) is an important quality indicator. The GPC of wheat grown in the middle and lower reaches of the Yangtze River is often low. Marker-assisted selection (MAS) is an effective tool for improving quantitative traits; however, most markers have not been effectively applied in GPC improvement except Gpc-B1, although many loci associated with GPC were identified. In this study, linkage analysis using a recombinant inbred line population from the cross of core parents of Ningmai 9 and Yangmai 158 and association mapping using the local cultivated varieties were performed and nine candidate intervals were identified. The appropriate kompetitive allele-specific PCR (KASP) markers associated with GPC were successfully developed and screened in 1163 F4 breeding lines. Three markers, Kgpc-2B, Kgpc-2D, and Kgpc-4A, were validated to be significantly related to GPC by large-scale association mapping, and they were combined to achieve the highest efficiency to enhance GPC. We applied these markers in 164 F6 breeding lines and obtained 15 lines with high GPC, indicating their high selective efficiency. Further, strategies for gene exploration in the three significant intervals were proposed. These results were expected to provide a novel route for improving GPC in wheat quality breeding.

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