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Identification of a TRIM32 from Penaeus monodon is involved in autophagy and innate immunity during white spot syndrome virus infection

文献类型： 外文期刊

作者： Peng, Chao 1 ; Zhao, Chao 1 ; Wang, Peng-Fei 1 ; Yan, Lu-Lu 1 ; Fan, Si-Gang 1 ; Qiu, Li-Hua 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Resources, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

5.Sanya Trop Fisheries Res Inst, Sanya, Hainan, Peoples R China

6.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Aquat Genom, Guangzhou, Guangdong, Peoples R China

关键词： Penaeus monodon; PmTRIM32; WSSV; Autophagy; Ubiquitination; Innate immunity

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2021 年 123 卷

页码：

收录情况： SCI

摘要： Many tripartite motif (TRIM) family proteins played an important role in regulating innate immune and autophagy pathway and were important for host defenses against viral pathogens. However, the role of TRIM proteins in autophagy and innate immunity during virus infection was seldom studied in crustaceans. In this study, a novel TRIM32 homolog was identified from Penaeus monodon (named PmTRIM32). PmTRIM32 was significantly upregulated by rapamycin stimulation and WSSV infection. RNA interference experiments showed that PmTRIM32 could restrict WSSV replication and lead P. monodon more resistance to WSSV challenge. Autophagy could be induced by WSSV or rapamycin challenge and has been proved to play a positive role in restricting WSSV replication in P. monodon. The autophagy activity induced by WSSV or rapamycin challenge could be obviously inhibited by silence of PmTRIM32 in P. monodon. Further studies revealed that PmTRIM32 positively regulated the expression of nuclear transcription factor (NF-kappa B) and it mediated antimicrobial peptides. Moreover, Pull-down and in vitro ubiquitination assay demonstrated that PmTRIM32 could interact with WSSV envelope protein and target it for ubiquitination in vitro. Collectively, this study demonstrated that PmTRIM32 restricted WSSV replication and was involved in positively regulating autophagy and NF-kappa B pathway during WSSV infection in P. monodon.

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