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The Adaptor Protein UvSte50 Governs Fungal Pathogenicity of Ustilaginoidea virens via the MAPK Signaling Pathway

文献类型： 外文期刊

作者： Cao, Huijuan 1 ; Gong, Hao 1 ; Song, Tianqiao 1 ; Yu, Mina 1 ; Pan, Xiayan 1 ; Yu, Junjie 1 ; Qi, Zhongqiang 1 ; Du, Yan 1 ; Liu, Yongfeng 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

关键词： rice false smut; Ustilaginoidea virens; adaptor protein UvSte50; MAPK signaling pathways; pathogenicity

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

ISSN：

年卷期： 2022 年 8 卷 9 期

页码：

收录情况： SCI

摘要： The mitogen-activated protein kinase (MAPK) signaling pathways regulate diverse cellular processes and have been partially characterized in the rice false smut fungus Ustilaginoidea virens. UvSte50 has been identified as a homolog to Saccharomyces cerevisiae Ste50, which is known to be an adaptor protein for MAPK cascades. Delta Uvste50 was found to be defective in conidiation, sensitive to hyperosmotic and oxidative stresses, and non-pathogenic. The mycelial expansion of Delta Uvste50 inside spikelets of rice terminated at stamen filaments, eventually resulting in a lack of formation of false smut balls on spikelets. We determined that UvSte50 directly interacts with both UvSte7 (MAPK kinase; MEK) and UvSte11 (MAPK kinase kinase; MEKK), where the Ras-association (RA) domain of UvSte50 is indispensable for its interaction with UvSte7. UvSte50 also interacts with UvHog1, a MAP kinase of the Hog1-MAPK pathway, which is known to have important roles in hyphal growth and stress responses in U. virens. In addition, affinity capture-mass spectrometry analysis and yeast two-hybrid assay were conducted, through which we identified the interactions of UvSte50 with UvRas2, UvAc1 (adenylate cyclase), and UvCap1 (cyclase-associated protein), key components of the Ras/cAMP signaling pathway in U. virens. Together, UvSte50 functions as an adaptor protein interacting with multiple components of the MAPK and Ras/cAMP signaling pathways, thus playing critical role in plant infection by U. virens.

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