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A Novel Bunyavirus Discovered in Oriental Shrimp (Penaeus chinensis)

文献类型： 外文期刊

作者： Dong, Xuan 1 ; Hu, Tao 2 ; Ren, Yanbei 1 ; Meng, Fanzeng 1 ; Li, Chen 1 ; Zhang, Qingli 1 ; Chen, Jiayuan 1 ; Song, Jipeng 1 ; Wang, Ruoyu 1 ; Shi, Mang 1 ; Li, Juan 2 ; Zhao, Peng 3 ; Li, Cixiu 2 ; Tang, Kathy F. J. 1 ; Cowley, Jeff A. 5 ; Shi, Weifeng 2 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst,Qingdao Key Lab Mar, Lab Marine Fisheries Sci & Food Prod Proc,Minist, Pilot Natl Lab Marine Sci & Technol Qingdao,Key L, Qingdao, Peoples R China

2.Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Etiol & Epidemiol Emerging Infect Dis, Tai An, Shandong, Peoples R China

3.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China

4.Sun Yat Sen Univ, Sch Med, Guangzhou, Peoples R China

5.CSIRO Agr & Food, Livestock & Aquaculture, Queensland Biosci Precinct, St Lucia, Qld, Australia

6.Ctr Asia Pacific, Network Aquaculture, Bangkok, Thailand

关键词： bunyavirus; Penaeus chinensis; Wenrivirus; Phenuiviridae; shrimp

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Herein, we describe a novel bunyavirus, oriental wenrivirus 1 (OWV1), discovered in moribund oriental shrimp (Penaeus chinensis) collected from a farm in China in 2016. Like most bunyaviruses, OWV1 particles were enveloped, spherical- to ovoid-shaped, and 80-115 nm in diameter. However, its genome was found to comprise four segments of (-)ssRNA. These included an L RNA segment (6,317 nt) encoding an RNA-directed RNA polymerase (RdRp) of 2,052 aa, an M RNA segment (2,978 nt) encoding a glycoprotein precursor (GPC) of 922 aa, an S1 RNA segment (1,164 nt) encoding a nucleocapsid (N) protein of 243 aa, and an S2 RNA segment (1,382 nt) encoding a putative non-structural (NSs2) protein of 401 aa. All the four OWV1 RNA segments have complementary terminal decanucleotides (5 '-ACACAAAGAC and 3 '-UGUGUUUCUG) identical to the genomic RNA segments of uukuviruses and similar to those of phleboviruses and tenuiviruses in the Phenuiviridae. Phylogenetic analyses revealed that the RdRp, GPC, and N proteins of OWV1 were closely related to Wenzhou shrimp virus 1 (WzSV-1) and Mourilyan virus (MoV) that infect black tiger shrimp (P. monodon). Phylogenetic analyses also suggested that OWV1 could be classified into a second, yet to be established, species of the Wenrivirus genus in the Phenuiviridae. These wenriviruses also clustered with Wenling crustacean virus 7 from shrimps and bunya-like brown spot virus from white-clawed crayfish. Of note there were no homologs of the NSs2 of OWV1 and MoV/WzSV-1 in GenBank, and whether other crustacean phenuiviruses also possess a similar S2 RNA segment warrants further investigation. In addition, we established a TaqMan probe-based reverse-transcription quantitative PCR method for detection of OWV1, and it was detected as 1.17 x 10(2)-1.90 x 10(7) copies/ng-RNA in gills of 23 out of 32 P. chinensis samples without an obvious gross sign. However, the discovery of OWV1 highlights the expanding genomic diversity of bunyaviruses.

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