文献类型： 会议论文

第一作者： Shiyong Gao

作者： Shiyong Gao ¹ ; Yubin Ji ¹ ; Chenfeng Ji ² ; Xiang Zou ³ ;

作者机构： 1.Postdoctoral Programme of the Institute of Materia, Medica, The Center for Life Sciences and Environmental Sciences, Harbin Commerce University

2.MOE Engineering Research Center of Natural, A nticancer Drugs, Harbin Commerce University

3.MOE Engineering Research Center of Natural, A nticancer Drugs, HarbinCommerce University

关键词： Solanine;HepG2;Apoptosis

会议名称： iCBBE 2009

主办单位：

页码： 1771-1774

摘要： The nightshade(Solanum nigrum Linn.) has been widely used in Chinese traditional medicine as a remedy for the treatment of digestive system cancer. The anti-tumor activity of solanine, a steroid alkaloid isolated from the nightshade has been demonstrated. To observe the effect of antitumor and mechanism of solanine. The MTT assay was used to evaluate the IC50 on the 3 digestive system tumor cell lines; The effect on the morphology was observed with a laser confocal microscopy; The rate of apoptosis and the cell cycle were measured using flow cytometry. The results show the IC{sub}50 for HepG2, SGC-7901, and LS-174 were 14.47, >50, and >50μg/ml, respectively; The morphology of cells in the negative control was normal; For the treated groups, typical signs for apoptosis were found. The rate of apoptosis in HepG2 cells induced by solanine was found to be 6.0%, 14.4%, 17.3%, 18.9%, and 32.2%, respectively. Observation of the cell cycle showed that cells in the G{sub}2/M phases disappeared while the number of cells in the S phase increased significantly for treated groups. Therefore, the solanine could induce apoptosis in HepG2 cells.

分类号： G202-53