文献类型： 会议论文

第一作者： Yubin JI

作者： Yubin JI ¹ ; Chenfeng JI ¹ ; Shiyong GAO ² ;

作者机构： 1.Institute of Materia Medica and Postdoctoral Programme Harbin University of Commerce

2.Engineering Reseach Center of Natural Anticancer Drugs Ministry of Education

关键词： Sargassum fusiforme;Polysaccharide;Erythrocyte;Ca~(2+)

会议名称： International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010)

主办单位：

页码： 2097-2102

摘要： To analyze the effect of Sargassum fusiforme polysaccharides (SFPS) on the functioning of erythrocyte membrane of S180 mice by affecting Ca~(2+) channels. A tumor mouse model was developed, and the mice were treated with high, medium, and low doses of SFPS. Changes in [Ca~(2+)] in the erythrocytes in the tumor-bearing mice were measured using laser confocal scanning; changes in the content of sialic acid and the acitivities of ATPase were meausred using relevant test kits; changes in the level of erythrocyte membrane potential were analyzed using FCM; and changes in the complex mobility of erythrocytes were measured using HPCE. SFPS lowered the intracelluar [Ca~(2+)] of erythrocytes in tumor-bearing mice, increased the content of sialic acid on surface of erythrocyte membrane, increased the acitivities of Na~+, K~+-ATPase and Ca~(2+), Mg~(2+)-ATPase, and raised the level of erythrocyte membrane potential and the complex mobility of erythrocytes in electrophoresis. By affecting the Ca~(2+) channels, SFPS can increase the content of sialic acid on the surface of erythrocyte membrane. The sialic acid combines with Ca~(2+) to increase the activities of Na~+, K~+-ATPase and Ca~(2+), Mg~(2+)-ATPase, resulting in a rise in the membrane potential of the erythrocytes and an increase in the complex mobility of the erythrocytes in electrophoresis, thus restoring many physiological and biochemical functions of the erythrocyte membrane. This may be one of the anti-tumor mechanisms of SFPS.

分类号： G202-53