您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A universal PCR primer for detection of members of the potyviridae

文献类型： 会议论文

第一作者： CHEN Jiong

作者： CHEN Jiong 1 ; CHEN Jian-ping 1 ; Michael J.Adams 2 ;

作者机构： 1.Virology Department, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China

2.Plant Pathology Department, IACR-Rothamsted, Harpenden, Herts, AL5, 2JQ, UK

会议名称： Proceedings:

主办单位：

页码： 108-

摘要： The Potyviridae is the largest of the plant virus families currently recognized. This family consists of 6 genera based on their transmission by fungi (Bymovirus), whiteflies (Ipomovirus), aphids (Macluravirus, Potyvirus) or mites (Rymovirus, Tritimovirus). All members have filamentous particles 650 approx 900 nm or 500 approx 600 and 200 approx 300 nm in length and 11 approx 13 nm in width, made up of a single structural coat protein surrounding a linear, single-stranded positive sense monopartite or bipartite RNA genome. All the viruses induce characteristic pinwheel or scrollshaped inclusion bodies in the cytoplasm of the infected cells. Because the genome organization of different members is similar, and some regions of genomes among different members are high conserved, it is possible to develop universal primers for use in RT-PCR to detect these viruses from infected plant materials. In this paper, a universal primer was designed from a highly conserved motif in the NIb domain and RT-PCR used to amplifty the 3'-terminal sequences of different members of th family. In addition, 3'-terminal sequences of oat necrotic mottle virus (ONMV) and celery mosaic virus (CeMV) were determined for the first time and a potyvirus infecting maize in China was identified.

分类号： S432.1

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台