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Study on Synthesis and Characterization of a Fluorescence Probe and Its Application for Determination of Superoxide Anion Radical

文献类型： 会议论文

第一作者： Yongqiang Zhao

作者： Yongqiang Zhao 1 ; Hong Lin 2 ; Laihao Li 1 ; Xiangqing Yang 2 ; Shuxian Hao 2 ; Jianwei Cen 2 ; Ya Wei 2 ; Bo Qi 2 ; Hui Huang 2 ;

作者机构： 1.Food Safety Laboratory, Ocean University of China, Qingdao, China

2.National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China

关键词： synthesis;characterization;fluorescence probe;superoxide anion radical

会议名称： International conference on biotechnology, chemical and materials engineering

主办单位：

页码： 1483-1487

摘要： A novel Schiff base,2-(Salicylideneamino)pyridine (2-SAP) synthesized by 2-aminopyridine and salicylaldehyde was used as a fluorescence probe to determine superoxide anion radical (O2·-) in this study.The synthetic product was characterized by IR,1H-NMR and GC-MS methods.A fluorescence quenching reaction occurs between 2-SAP and O2·-,and the fluorescence intensity decreased significantly.The effect of pyrogallol concentration (2.0× 10-5M to l.0× 10-3 M) was discussed in this study.In the range from 2.0×10-5 M to 1.0×l0-4 M,relative fluorescence intensity (y) and pyrogallol concentration (x) shown a good linear relationship,y=37.801x+76.854,r=0.993.Based on this phenomenon,the O2·-generated by pyrogallol autoxidation could be determined simply with high sensitivity.

分类号： q81

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