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Identification of Symbiotic or Epiphyte Microbe F17 from Pinctada Fucata and Analysis of Its Main Metabolites by GC-MS

文献类型: 会议论文

第一作者: Yanyan Wu

作者: Yanyan Wu 1 ; Yan Zhang 1 ; Laihao Li 1 ; Xianqing Yang 1 ; Wei Shi 2 ; Zhengyi Shao 3 ;

作者机构: 1.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, People's Republic of China,National R&D Center For Aquatic Product Processing, Guangzhou 510300, People's Republic of China,Key Lab of Aquatic Product Pro

2.Guangdong Huankai Microbial Science and Technology Co., Ltd, Guangzhou 510663, People's Republic of China

3.College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China

关键词: Bacillus flexus;Identification;GC-MS;Extracellular metabolites

会议名称: International conference on applied biotechnology

主办单位:

页码: 1291-1300

摘要: The strain F17 which had broad antibacterial spectrum and strong antibacterial activity was isolated from Pinctada fucata sample collected from Sanya, China. This strain was finally identified as Bacillus flexus based on morphological, biochemical characteristics, and 16S rRNA sequence. Antifungal spectrum was confirmed by oxford piate assay system and showed that F17 had significant inhibitory effect against Escherichia coli, Bacillus subtilis, Staphylo-coccus aureus, Listeria monocytogenes, Pseudomoas aerunginosa, Salmonella typhimurium, Vibrio parahaemolyticus, Shigella dysenteriae, etc. The volatilis components of the extracellular metabolites of F17 were analyzed by GC-MS. The results showed that the main chemical compositions were 2-Methyl butyric acid, Dioctyl phthalate, 2,3-Butanediol, 4-Chloro-2-nitrobenzyl alcohol, and Glycyl-L-proline in the fermentation fluid extracted by ethyl acetate. In those, 2-Methyl butyric acid and 2,3-Butanediol can be used in food flavor; 4-Chloro-2-nitrobenzyl alcohol has certain development potential for antiseptic. Glycyl-L-proline is an important raw material in pharmaceutical and nutrient food industry. The experiments showed the metabolites of F17 had great potential as food additives.

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