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Evaluation of antioxidant activity of black-seeded wheat

文献类型: 会议论文

第一作者: T. Beta

作者: T. Beta 1 ; W. Li 2 ; S. Sun 3 ; H. Corke 4 ;

作者机构: 1.University of Manitoba, Winnipeg, Manitoba, Canada

2.Shanxi University, Taiyuan City, P.R. China

3.Institute of Crop Genetics, Shanxi Academy of Agricultural Sciences, Taiyuan City, P.R. China

4.Department of Botany, University of Hong Kong, Hong Kong

会议名称: Annual Meeting of AACC International

主办单位:

页码: 324-

摘要: Natural antioxidants have gained great attention in recent years for their role in prevention of many diseases, such as atherosclerosis and heart disease. It is proved that wheat bran contains rich natural antioxidants. In the present study, the antioxidant activity of wheat, comprised of a black seed coat color, was evaluated. Black seed wheat belongs to a new genotype that has been successfully bred in a Chinese breeding programme for over 20 years. Cereal nutrient composition has a close relationship with its seed color. Our previous study found that black seed wheat contained a high protein content of over 17%. Our present research proves that the bran of black seed wheat also contains very high antioxidant activity. Four wheat genotypes were compared on their ability to scavenge DPPH radicals. Radical scavenging capacity of bran extracts was 70.87% for black-, 67.59% for Dongjian brown-, and 49.64% for blue-seeded wheat. Radical scavenging capacity of whole grain flour extracts was 33.1% for black-, 25.59% for Dongjian brown-, 25.46% for Dongjian white- and 23.68% for blue-seeded wheat. Reaction kinetics of bran extracts with DPPH radical monitored for 60 min indicated that there were significant differences among three wheat genotypes. Lowest DPPH remaining (34.09%) of bran extract from black-seeded wheat at 60 min indicated that it had rapid rate of scavenging ability. High performance liquid chromatographic (HPLC) assay further showed that seed coat color affected phenolic antioxidant composition of wheat bran. Our results are valuable for developing new uses of black-seeded wheat, and suggest that the bran may serve as an excellent dietary source of natural antioxidants for disease prevention and health promotion.

分类号: TS210.1-794

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